Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China.
The Second People's Hospital of Heze in Shandong Province, Heze 274005, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004794.
A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2, was isolated from a surface-sterilized branch of collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China. Strain CMS6Z-2 grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %). Strain CMS6Z-2 possessed -diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H) as the predominant menaquinone. The major fatty acids were iso-C, C and C 9. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The G+C content of the genomic DNA was 74.1 mol%. Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2 should be assigned to the genus and its closest relative was IP6SC6 with 98.3 % similarity. Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2 belonged to the genus and formed a robust cluster with IP6SC6 within the genus . The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2 and the type strain of were 81.5 and 23.9 %, respectively. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CMS6Z-2 (=KCTC 49240=CGMCC4.7549).
一株革兰氏阳性、需氧、非运动、非芽孢形成和杆状放线菌，被命名为 CMS6Z-2 菌株，从中国广西壮族自治区涠洲岛采集的经过表面消毒的树枝中分离得到。CMS6Z-2 菌株在 10-37°C（最佳温度为 37°C）、pH6.0-9.0（最佳 pH 值为 7.0-8.0）和 0-10.0%（w/v）NaCl 存在下生长（最佳为 0-1.0%）。CMS6Z-2 菌株的肽聚糖含有二氨基庚二酸，主要的醌为 MK-8（H）。其主要脂肪酸为 iso-C 15:0、C 16:0 和 C 17:0 9。极性脂类包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和两种未知的磷脂。基因组 DNA 的 G+C 含量为 74.1mol%。16S rRNA 基因的比较分析表明，CMS6Z-2 菌株应归属于 属，其最接近的亲缘种是 IP6SC6，相似度为 98.3%。基于 16S rRNA 基因序列的系统发育分析和基于核心蛋白组比对的系统发育分析表明，CMS6Z-2 菌株属于 属，与 IP6SC6 形成一个紧密的分支，属于 属。CMS6Z-2 菌株与 模式菌株之间的平均核苷酸同一性值和估计的数字 DNA-DNA 杂交值分别为 81.5%和 23.9%。基于系统发育、表型和化学分类特征，CMS6Z-2 菌株代表了一个新的 属种，命名为 sp. nov.。其模式菌株为 CMS6Z-2（=KCTC 49240=CGMCC 4.7549）。
