sp. nov., a novel endophytic actinobacterium isolated from branch of .

A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2, was isolated from a surface-sterilized branch of collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China. Strain CMS6Z-2 grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %). Strain CMS6Z-2 possessed -diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H) as the predominant menaquinone. The major fatty acids were iso-C, C and C 9. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The G+C content of the genomic DNA was 74.1 mol%. Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2 should be assigned to the genus and its closest relative was IP6SC6 with 98.3 % similarity. Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2 belonged to the genus and formed a robust cluster with IP6SC6 within the genus . The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2 and the type strain of were 81.5 and 23.9 %, respectively. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CMS6Z-2 (=KCTC 49240=CGMCC4.7549).