Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Sep;114(9):1431-1442. doi: 10.1007/s10482-021-01614-7. Epub 2021 Jul 15.
A novel endophytic actinomycete, designated strain BSK3Z-2, was isolated from a surface-sterilised branch of Avicennia mariana from Shankou Mangrove Nature Reserve, Guangxi Zhuang Autonomous Region, China. Cells were observed to be Gram-stain positive, aerobic, asporogenous and rod-shaped. Strain BSK3Z-2 was found to grow optimally at 30 °C, pH 7.0 and in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BSK3Z-2 belongs to the genus Phycicoccus and has high 16S rRNA gene sequence similarity of 98.1% with Phycicoccus endophyticus IP6SC6. Phylogenetic analysis based on the genome of strain BSK3Z-2 was performed by extracting and aligning 39 conserved proteins and 88 housekeeping genes, which further confirmed the phylogenetic assignment of strain BSK3Z-2. The draft genome of strain BSK3Z-2 is 3.54 Mbp with a DNA G + C content of 73.8%. The average nucleotide identity (ANI), amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strain BSK3Z-2 and species of genus Phycicoccus were 73.8-85.6%, 64.5-75.9% and 19.5-23.8%, respectively, which are below the standard cut-off values for bacterial species delineation. Strain BSK3Z-2 contains MK-8(H) as the dominant menaquinone. The cell wall peptidoglycan contains meso-diaminopimelic acid. The polar lipids profile of strain BSK3Z-2 was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant fatty acids were identified as C, C, iso-C and Cω8c. Comparing the phenotypic and phylogenetic features of the strain BSK3Z-2 and related taxa, strain BSK3Z-2 is concluded to represent a novel species of the genus Phycicoccus, for which the name Phycicoccus avicenniae sp. nov. is proposed. The type strain is BSK3Z-2 (= CGMCC 1.18743 = JCM 34335).
一株新的内生放线菌，菌株 BSK3Z-2，从广西壮族自治区山口红树林自然保护区经表面消毒的红海榄树枝上分离得到。观察到细胞革兰氏阳性、需氧、无孢子和杆状。BSK3Z-2 菌株在 30°C、pH7.0 和 1%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 BSK3Z-2 属于 Phycicoccus 属，与 Phycicoccus endophyticus IP6SC6 的 16S rRNA 基因序列相似性高达 98.1%。基于菌株 BSK3Z-2 基因组提取和比对 39 个保守蛋白和 88 个看家基因进行的系统发育分析，进一步证实了菌株 BSK3Z-2 的系统发育归属。BSK3Z-2 菌株的基因组草图为 3.54 Mbp，GC 含量为 73.8%。BSK3Z-2 菌株与 Phycicoccus 属种之间的平均核苷酸同一性 (ANI)、氨基酸同一性 (AAI) 和数字 DNA-DNA 杂交 (dDDH) 值分别为 73.8-85.6%、64.5-75.9%和 19.5-23.8%，均低于细菌种划分的标准截止值。BSK3Z-2 菌株含有 MK-8(H)作为主要的menaquinone。细胞壁肽聚糖含有 meso-二氨基庚二酸。BSK3Z-2 菌株的极性脂谱分析发现含有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇。主要脂肪酸为 C16:0、C18:1ω7c 和 iso-C15:0。比较菌株 BSK3Z-2 与相关分类群的表型和系统发育特征，认为菌株 BSK3Z-2 代表 Phycicoccus 属的一个新种，命名为 Phycicoccus avicenniae sp. nov.。模式菌株为 BSK3Z-2(=CGMCC 1.18743=JCM 34335)。
