Tibet Plateau Institute of Biology, Lhasa, Tibet 850001, PR China.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004791.
A Gram-stain-negative, aerobic, flagellated, non-spore-forming, rod-shaped bacterium, named B2R-29, was isolated from water collected from a crater lake on Da Hinggan mountain, PR China. Strain B2R-29 was oxidase- and catalase-positive. On the basis of the results of 16S rRNA gene sequence analyses, strain B2R-29 clearly belonged to the family , class and showed the highest similarity to EM1 (97.4 %) and to the other species of (less than 96.8 %). In the phylogenetic tree, strain B2R-29 formed a clade with EM1 and CMJ-9, indicating that is a member of the genus . Digital DNA-DNA hybridization and average nucleotide identity analyses were performed and the values between strain B2R-29 and its closely related species were less than 75.1 % and 16.9 %, respectively. The chemotaxonomic data of B2R-29 were as follows: major uniquinone, Q-8; predominant polar lipids, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol; major fatty acids, C and summed feature 3 (C 7 / C 6); predominant polyamines, putrescine, 2-hydroxyputrescine and spermidine. The DNA G+C content was 51.7 mol% from the genomic sequencing data. In accordance with the phenotypic, physiological and chemotaxonomic properties mentioned above, strain B2R-29 represents a novel species of the genus for which the name sp. nov is proposed. The type strain is B2R-29 (=CGMCC 1.13792=KCTC 72018).
一株革兰氏阴性、需氧、鞭毛、不产芽孢的杆状细菌，被命名为 B2R-29，从中国大兴安岭火山口湖采集的水样中分离得到。B2R-29 菌株氧化酶和过氧化氢酶均为阳性。基于 16S rRNA 基因序列分析的结果，B2R-29 菌株明显属于 科、 纲和 目，与 EM1（97.4%）和其他 属的种的相似度最高（低于 96.8%）。在系统发育树中，B2R-29 菌株与 EM1 和 CMJ-9 形成一个分支，表明它是 属的一个成员。进行了数字 DNA-DNA 杂交和平均核苷酸同一性分析，B2R-29 菌株与其密切相关的种之间的数值分别小于 75.1%和 16.9%。B2R-29 的化学分类数据如下：主要醌，Q-8；主要极性脂，磷脂酰乙醇胺、双磷脂酰甘油和磷脂酰甘油；主要脂肪酸，C 和特征脂肪酸 3（C 7 / C 6）；主要多胺，腐胺、2-羟腐胺和亚精胺。根据基因组测序数据，DNA G+C 含量为 51.7mol%。根据上述表型、生理和化学分类特征，B2R-29 菌株代表了 属的一个新种，建议将其命名为 sp. nov。其模式菌株是 B2R-29（=CGMCC 1.13792=KCTC 72018）。
