Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
Biological Resources Utilization Department, National Institute of Biological Resources, Incheon, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3148-3154. doi: 10.1099/ijsem.0.003604.
A novel Gram-stain-negative, non-spore-forming, obligate aerobic, motile, rod-shaped, and flagellated bacterium, designated S11R28, was isolated from the intestinal tract of a Korean shiner, . Based on 16S rRNA gene sequences, strain S11R28 was identified as member of the genus in class , and was closely related to DSM 23061 (98.49 %). The isolate grew at 4-25 °C, pH 6-9, with 0 % (w/v) NaCl, and grew optimally at 20 °C, pH 8, in the absence of NaCl. The main cellular fatty acids were C and summed features 3 (Cω7 and/or Cω6). The strain possessed diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as predominant polar lipids, and ubiquinone Q-8 as a respiratory quinone. The polyamine profile composed of 2-hydroxyputrescine, spermidine, putrescine, and benzoic acid. A genomic DNA G+C content was 51.4 mol%. The average nucleotide identity between strains S11R28 and DSM 23061 was 78.66 %. Thus, can be considered a novel species within the genus with the type strain S11R28 (=KCTC 62668=JCM 33224).
一株新型革兰氏阴性、非孢子形成、需氧、能动、杆状、具鞭毛的细菌，命名为 S11R28，从韩国青鳉的肠道中分离得到。根据 16S rRNA 基因序列，菌株 S11R28 被鉴定为属的成员，在纲中，与 DSM 23061（98.49%）最为密切相关。该分离株在 4-25°C、pH6-9、0%（w/v）NaCl 下生长，在无 NaCl 的情况下最适生长温度为 20°C、pH8。主要细胞脂肪酸为 C 和特征脂肪酸 3（Cω7 和/或 Cω6）。该菌株具有双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂，以及醌-8 作为呼吸醌。多胺图谱由 2-羟基腐胺、腐胺、亚精胺和苯甲酸组成。基因组 DNA G+C 含量为 51.4mol%。菌株 S11R28 和 DSM 23061 之间的平均核苷酸同一性为 78.66%。因此，可被认为是属内的一个新种，其模式菌株为 S11R28（=KCTC 62668=JCM 33224）。
