Universidade Federal da Grande Dourados, Faculdade de Ciências e Tecnologia, Rodovia Dourados/Ithaum, Unidade Km 12-2, Caixa Postal 364, 79804-970 Dourados, MS, Brazil.
Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Instituto de Física, Caixa Postal 549, 79070-900 Campo Grande, MS, Brazil.
An Acad Bras Cienc. 2021 Apr 26;93(3):e20190739. doi: 10.1590/0001-3765202120190739. eCollection 2021.
Nanotechnology is a field that, over the years, has been growing in several research areas, such as medicine, agriculture and cosmetics, among others. As a result, there is a continuous increase in the production, use and disposal of these materials in the environment. The behaviour and (bio) activity of these materials in the atmosphere, water and soil are not fully studied. Therefore, it is necessary to carry out an analysis of the risks of contamination, as well as the possible effects and impacts of nanoparticles (NPs) on the ecosystem. In an attempt to investigate these effects on plants, the present study aimed to investigate the impact of copper oxide nanoparticles (CuO NPs) on the seed germination process of Sesbania virgata. For this, the Sesbania virgata seeds were subjected to different concentration of CuO NPs (0, 100, 200, 300 and 400 mgL-1) and their germination and development were monitored by optical analysis (thermography and chlorophyll a fluorescence). The results show that the CuO NPs induced a reduction on the maximum emission of chlorophyll a, which was concentration-dependent. The data also showed that CuO NPs promoted an increase in the energy dissipated by non-photochemical pathways and the surface temperature of the seeds. Additionally, our findings revealed that CuO NPs caused a root growth inhibition. In summary, the present study demonstrates, for the first time, that CuO NPs can negatively affect the physiological status and development of the S. virgata plant, by altering the efficiency of the functioning of photosystem II in its initial developmental stage, depending on the concentration of CuO NPs.
纳米技术是一个多年来在多个研究领域（如医学、农业和化妆品等）不断发展的领域。因此，这些材料在环境中的生产、使用和处置不断增加。这些材料在大气、水和土壤中的行为和（生物）活性尚未得到充分研究。因此，有必要对污染风险以及纳米颗粒（NPs）对生态系统的可能影响和影响进行分析。为了试图研究这些对植物的影响，本研究旨在研究氧化铜纳米颗粒（CuO NPs）对田菁种子萌发过程的影响。为此，将田菁种子暴露于不同浓度的 CuO NPs（0、100、200、300 和 400 mgL-1）中，并通过光学分析（热成像和叶绿素 a 荧光）监测其萌发和发育。结果表明，CuO NPs 诱导了叶绿素 a 最大发射的降低，这是浓度依赖性的。数据还表明，CuO NPs 促进了非光化学途径的能量耗散和种子表面温度的增加。此外，我们的研究结果表明，CuO NPs 导致根生长受到抑制。总之，本研究首次表明，CuO NPs 可以通过改变光合作用系统 II 在其初始发育阶段的功能效率，对 S. virgata 植物的生理状态和发育产生负面影响，这取决于 CuO NPs 的浓度。
