Westergren I, Johansson B B
Department of Neurology, University Hospital, Lund, Sweden.
Brain Res Bull. 1991 Aug;27(2):283-6. doi: 10.1016/0361-9230(91)90083-v.
Although rat cerebrospinal fluid (CSF) is increasingly being used in pharmacological and biochemical research, methodological studies on basic physiological data are lacking. We have determined the albumin content and number of erythrocytes and leukocytes in CSF obtained by two different methods of sampling from cisterna magna-repeated sampling from an implanted cannula and by repeated punctures. In the initial samples the albumin content was 0.08 +/- 0.03 micrograms/microliters. Chronic cannulation of the cisterna magna resulted in a meningeal reaction with increased cell and albumin content: a reaction that could be reduced but not prevented by using a sterile cannula. The number of leukocytes but not erythrocytes was highly correlated to the albumin content. Repeated sampling in the absence of a permanent cannula did not significantly increase albumin content but carried a higher risk for erythrocyte contamination.
尽管大鼠脑脊液（CSF）越来越多地用于药理学和生物化学研究，但缺乏关于基本生理数据的方法学研究。我们测定了通过两种不同的采样方法从枕大池获取的脑脊液中的白蛋白含量、红细胞和白细胞数量，这两种方法分别是通过植入套管重复采样和重复穿刺。在初始样本中，白蛋白含量为0.08±0.03微克/微升。枕大池长期插管会导致脑膜反应，细胞和白蛋白含量增加：使用无菌套管可减轻但不能预防这种反应。白细胞数量而非红细胞数量与白蛋白含量高度相关。在没有永久性套管的情况下重复采样不会显著增加白蛋白含量，但红细胞污染风险更高。
