The Ninth People's Hospital of Shanghai, Jiao Tong University School of Medicine, Shanghai 200011, PR China.
The Ninth People's Hospital of Shanghai, Jiao Tong University School of Medicine, Shanghai 200011, PR China.
Ecotoxicol Environ Saf. 2021 Jul 1;217:112259. doi: 10.1016/j.ecoenv.2021.112259. Epub 2021 Apr 25.
Surgical smoke is widespread in operating rooms, and fine particles are the main toxic components. However, the effect of fine particles in surgical smoke on embryonic development has not yet been studied. This study evaluated the effect of fine particles in surgical smoke on embryonic development and compared it with that of atmospheric fine particles. Afterwards, differentiated cardiomyocytes were purified, and the effect of exposure to fine particles in surgical smoke on cardiomyocyte differentiation was evaluated. Fine particles in surgical smoke exhibited weak embryotoxicity toward an embryonic stem cell test model, and their inhibitory effect on cardiomyocyte differentiation was slightly stronger than that of atmospheric fine particles. Fine particles in surgical smoke specifically inhibited the differentiation of the mesoderm lineage and promoted the differentiation of the ectoderm lineage. Furthermore, fine particles in surgical smoke reduced the beating rate of purified cardiomyocytes, promoted mitophagy, reduced ATP production and increased the reactive oxygen species (ROS) content. Antioxidants attenuated the inhibition of cardiomyocyte differentiation and the reduction in the cardiomyocyte beating rate caused by fine particles in surgical smoke and simultaneously restored mitophagy and other processes to the control levels. However, mitophagy inhibitors treatment blocked only the inhibition of cardiomyocyte differentiation caused by fine particles in surgical smoke; it had little effect on other changes caused by fine particles. Based on the results described above, we propose that fine particles in surgical smoke and atmospheric fine particles exhibit similar levels of toxicity toward embryonic development. Fine particles in surgical smoke potentially affect the beating of cardiomyocytes by damaging mitochondria and increasing oxidative stress.
手术烟雾广泛存在于手术室中，其中细颗粒物是主要的有毒成分。然而，手术烟雾中的细颗粒物对胚胎发育的影响尚未得到研究。本研究评估了手术烟雾中的细颗粒物对胚胎发育的影响，并将其与大气细颗粒物进行了比较。随后，对分化的心肌细胞进行纯化，并评估了暴露于手术烟雾中的细颗粒物对心肌细胞分化的影响。手术烟雾中的细颗粒物对胚胎干细胞测试模型表现出较弱的胚胎毒性，其对心肌细胞分化的抑制作用略强于大气细颗粒物。手术烟雾中的细颗粒物特异性地抑制中胚层谱系的分化，并促进外胚层谱系的分化。此外，手术烟雾中的细颗粒物降低了纯化的心肌细胞的跳动频率，促进了自噬，减少了 ATP 的产生，并增加了活性氧（ROS）的含量。抗氧化剂减弱了手术烟雾中的细颗粒物对心肌细胞分化的抑制作用和对心肌细胞跳动频率的降低作用，同时将自噬等过程恢复到对照水平。然而，自噬抑制剂处理仅阻断了手术烟雾中的细颗粒物对心肌细胞分化的抑制作用；它对细颗粒物引起的其他变化几乎没有影响。基于上述结果，我们提出手术烟雾中的细颗粒物和大气细颗粒物对胚胎发育具有相似的毒性。手术烟雾中的细颗粒物可能通过损伤线粒体和增加氧化应激来影响心肌细胞的跳动。
