条件重编程细胞培养中循环胃肠癌细胞的检测。
Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture.
机构信息
Department of General Surgery, Medical School, Daegu Catholic University Medical Center, Daegu, Republic of Korea.
Department of General Surgery, Medical School, Yonsei University, Seoul, Republic of Korea.
出版信息
In Vivo. 2021 May-Jun;35(3):1515-1520. doi: 10.21873/invivo.12404.
BACKGROUND/AIM: The aim of this study was to detect circulating tumor cells (CTC) in the peripheral blood of gastrointestinal cancer patients using conditionally reprogrammed cell (CRC) culture.
MATERIALS AND METHODS
We confirmed the sensitivity of the CRC culture method. Five ml of blood were obtained from 81 cancer patients (56 colorectal and 25 gastric). The collected mononuclear cells were cultured for 4 weeks in the CRC condition. Finally, cultured cells were characterized by RT-PCR for the expression of hTERT and MAGE A1-6 mRNA.
RESULTS
The CRC method had a CTC detection limit of 6 cells for gastric cancer cells. After culture of 81 blood specimens, 38 formed visible cells, including 5 colonies. Among the 38 cells, 13 were hTERT positive and 4 were MAGE A1-6 positive. The final CTC detection rate was 16.0%.
CONCLUSION
The CRC culture may potentially be used to evaluate the metastatic cancer cells in the circulation.
背景/目的：本研究旨在使用条件重编程细胞（CRC）培养法检测胃肠道癌患者外周血中的循环肿瘤细胞（CTC）。
材料和方法
我们证实了 CRC 培养法的敏感性。从 81 例癌症患者（56 例结直肠癌和 25 例胃癌）中采集 5ml 血液。收集的单核细胞在 CRC 条件下培养 4 周。最后，通过 RT-PCR 对培养细胞中 hTERT 和 MAGE A1-6 mRNA 的表达进行特征分析。
结果
CRC 方法对胃癌细胞的 CTC 检测限为 6 个细胞。培养 81 份血样后，形成了 38 个可见细胞，包括 5 个菌落。在这 38 个细胞中，有 13 个 hTERT 阳性，4 个 MAGE A1-6 阳性。最终的 CTC 检测率为 16.0%。
结论
CRC 培养法可能有潜力用于评估循环中的转移性癌细胞。
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