Jeong Young Ju, Park Sung Hwan, Jeon Chang-Ho
Department of Surgery, Catholic University of Daegu School of Medicine, Daegu 42472, Republic of Korea.
Department of Laboratory Medicine, Catholic University of Daegu School of Medicine, Daegu 42472, Republic of Korea.
Oncol Lett. 2020 Oct;20(4):78. doi: 10.3892/ol.2020.11939. Epub 2020 Jul 31.
The present study aimed to verify the efficacy of the conditionally reprogrammed cell (CRC) culture method for the detection of circulating tumor cells (CTCs) in breast cancer. CTCs were isolated from the peripheral blood of patients with breast cancer, and culture of the collected CTCs was performed according to the conditional reprogramming protocol. Total RNA was extracted from cultured CTCs, and the and genes were amplified using reverse transcription-PCR (RT-PCR). In addition, RNA extraction from another blood sample was performed and the expression of the two genes was analyzed by RT-PCR only. Following CRC culture, grown CTCs were observed in 7 samples (23.3%). The CTC detection rates by RT-PCR for the and genes in CTCs grown using the CRC culture method were 26.7 and 10.0%, respectively. The positive expression rates for the and genes in CTCs assessed by RT-PCR only were 44.1 and 23.5%, respectively. When combining the positive expression rates of RT-PCR only and CRC culture for the and genes, CTC detection rates increased to 53.3 and 23.3%, respectively. Additionally, when combining the positive expression rates of the two genes by either method, the CTC detection rate was the highest value observed. In conclusion, the present study revealed the potential of CRC culture in the detection of CTCs in breast cancer. Furthermore, a combination of CRC culture and RT-PCR for the and genes is useful in enhancing the detection rate of CTCs in the blood.
本研究旨在验证条件重编程细胞（CRC）培养方法用于检测乳腺癌循环肿瘤细胞（CTC）的有效性。从乳腺癌患者的外周血中分离出CTC，并根据条件重编程方案对收集到的CTC进行培养。从培养的CTC中提取总RNA，使用逆转录聚合酶链反应（RT-PCR）扩增 和 基因。此外，从另一血液样本中提取RNA，仅通过RT-PCR分析这两个基因的表达。在CRC培养后，在7个样本（23.3%）中观察到有生长的CTC。使用CRC培养方法培养的CTC中，通过RT-PCR检测 和 基因的CTC检出率分别为26.7%和10.0%。仅通过RT-PCR评估的CTC中， 和 基因的阳性表达率分别为44.1%和23.5%。当将仅RT-PCR和CRC培养对 和 基因的阳性表达率相结合时，CTC检出率分别提高到53.3%和23.3%。此外，当通过任一种方法将两个基因的阳性表达率相结合时，CTC检出率为观察到的最高值。总之，本研究揭示了CRC培养在检测乳腺癌CTC方面的潜力。此外，CRC培养与针对 和 基因的RT-PCR相结合有助于提高血液中CTC的检出率。
