Thiel J, Koppolu R, Trautewig C, Hertig C, Kale S M, Erbe S, Mascher M, Himmelbach A, Rutten T, Esteban E, Pasha A, Kumlehn J, Provart N J, Vanderauwera S, Frohberg C, Schnurbusch T
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, OT Gatersleben, 06466 Seeland, Germany.
Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada.
Sci Adv. 2021 Apr 28;7(18). doi: 10.1126/sciadv.abf0832. Print 2021 Apr.
Organ development in plants predominantly occurs postembryonically through combinatorial activity of meristems; therefore, meristem and organ fate are intimately connected. Inflorescence morphogenesis in grasses (Poaceae) is complex and relies on a specialized floral meristem, called spikelet meristem, that gives rise to all other floral organs and ultimately the grain. The fate of the spikelet determines reproductive success and contributes toward yield-related traits in cereal crops. Here, we examined the transcriptional landscapes of floral meristems in the temperate crop barley ( L.) using RNA-seq of laser capture microdissected tissues from immature, developing floral structures. Our unbiased, high-resolution approach revealed fundamental regulatory networks, previously unknown pathways, and key regulators of barley floral fate and will equally be indispensable for comparative transcriptional studies of grass meristems.
植物器官发育主要在胚胎后阶段通过分生组织的组合活动发生；因此，分生组织和器官命运紧密相连。禾本科植物的花序形态发生复杂，依赖于一种特殊的花分生组织，称为小穗分生组织，它产生所有其他花器官并最终形成籽粒。小穗的命运决定了生殖成功，并对谷类作物的产量相关性状有贡献。在这里，我们使用激光捕获显微切割技术从发育中的未成熟花结构组织进行RNA测序，研究了温带作物大麦（Hordeum vulgare L.）花分生组织的转录图谱。我们无偏见的高分辨率方法揭示了基本调控网络、以前未知的途径以及大麦花命运的关键调节因子，对于禾本科分生组织的比较转录研究同样不可或缺。
