柳枝稷基因组的染色体水平组装和分析。
Chromosome-scale assembly and analysis of biomass crop Miscanthus lutarioriparius genome.
机构信息
National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200233, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
出版信息
Nat Commun. 2021 Apr 28;12(1):2458. doi: 10.1038/s41467-021-22738-4.
Miscanthus, a rhizomatous perennial plant, has great potential for bioenergy production for its high biomass and stress tolerance. We report a chromosome-scale assembly of Miscanthus lutarioriparius genome by combining Oxford Nanopore sequencing and Hi-C technologies. The 2.07-Gb assembly covers 96.64% of the genome, with contig N50 of 1.71 Mb. The centromere and telomere sequences are assembled for all 19 chromosomes and chromosome 10, respectively. Allotetraploid origin of the M. lutarioriparius is confirmed using centromeric satellite repeats. The tetraploid genome structure and several chromosomal rearrangements relative to sorghum are clearly demonstrated. Tandem duplicate genes of M. lutarioriparius are functional enriched not only in terms related to stress response, but cell wall biosynthesis. Gene families related to disease resistance, cell wall biosynthesis and metal ion transport are greatly expanded and evolved. The expansion of these families may be an important genomic basis for the enhancement of remarkable traits of M. lutarioriparius.
芦竹是一种根茎多年生植物，具有生物能源生产的巨大潜力，因为它具有高生物质和耐胁迫能力。我们通过结合牛津纳米孔测序和 Hi-C 技术，报道了芦竹的染色体水平基因组组装。2.07Gb 的组装覆盖了基因组的 96.64%，串联群 N50 为 1.71Mb。着丝粒和端粒序列分别组装到了所有 19 条染色体和 10 号染色体上。使用着丝粒卫星重复序列证实了芦竹的同源四倍体起源。与高粱相比，四倍体基因组结构和几个染色体重排清晰可见。芦竹的串联重复基因不仅在与胁迫反应相关的方面，而且在细胞壁生物合成方面也具有功能富集。与疾病抗性、细胞壁生物合成和金属离子转运相关的基因家族得到了极大的扩展和进化。这些家族的扩张可能是芦竹显著性状增强的重要基因组基础。
Zotero 插件