Martinez C, Zumalacárregui J M, Diez V
Departamento de Bioquímica y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de León, España.
Enzyme. 1988;39(4):199-204. doi: 10.1159/000469119.
Adenosine deaminase from bovine skeletal muscle catalyzes the hydrolytic deamination of adenosine to inosine and ammonia via an ordered Uni-Bi mechanism, if water is not considered as a true second substrate, as deduced from the inhibition pattern products. The inhibition constants (Ki) obtained for inosine and ammonia were 316 mumol/l and 2 mol/l, respectively. The activation energy of the reaction has been calculated as 10 kcal/mol, delta H* and delta F* as 7.9 and 15.6 kcal/mol, respectively, and delta S* as -23 cal/mol/degrees K.
