Koizumi H, Iizuka H, Aoyagi T, Miura Y
Arch Dermatol Res. 1983;275(5):310-4. doi: 10.1007/BF00417203.
Adenosine deaminase, which catalyzes the irreversible hydrolytic deamination of adenosine and deoxyadenosine to inosine and deoxyinosine respectively, plays an important role in the degradation of adenine nucleotide and purine nucleotide salvage pathway metabolism. We investigated human epidermal adenosine deaminase activity using a radiochemical method, which enabled us to measure the adenosine deaminase activity of protein samples as small as several micrograms. We measured adenosine deaminase activity of microdissected pure epidermis of the healthy skin and the psoriatic affected and unaffected skin. It was shown that psoriatic affected epidermis had increased adenosine deaminase activity compared with the healthy epidermis (P less than 0.05) and the unaffected epidermis (P less than 0.01). There was no difference in enzyme activity between healthy and psoriatic unaffected epidermis. The increased adenosine deaminase activity in the psoriatic affected epidermis may reflect the accelerated salvage pathway of the nucleic acid metabolism probably associated with the hyperproliferative condition of the psoriatic epidermis.
腺苷脱氨酶分别催化腺苷和脱氧腺苷不可逆地水解脱氨生成肌苷和脱氧肌苷，在腺嘌呤核苷酸降解及嘌呤核苷酸补救途径代谢中起重要作用。我们采用放射化学方法研究了人表皮腺苷脱氨酶活性，该方法能让我们测定低至几微克蛋白质样品的腺苷脱氨酶活性。我们测量了健康皮肤、银屑病患部皮肤及未患部皮肤经显微切割后的纯表皮的腺苷脱氨酶活性。结果显示，与健康表皮（P<0.05）及未患部表皮（P<0.01）相比，银屑病患部表皮的腺苷脱氨酶活性增加。健康表皮与银屑病未患部表皮之间的酶活性无差异。银屑病患部表皮中腺苷脱氨酶活性增加可能反映了核酸代谢补救途径的加速，这可能与银屑病表皮的过度增殖状态有关。
