布鲁姆综合征细胞系GM1492中一种DNA连接酶刺激因子的表达。

Expression of a DNA-ligase-stimulatory factor in Bloom's syndrome cell line GM1492.

作者信息

Kenne K, Ljungquist S

机构信息

Department of Medical Cell Genetics, Karolinska Institutet, Stockholm, Sweden.

出版信息

Eur J Biochem. 1988 Jun 15;174(3):465-70. doi: 10.1111/j.1432-1033.1988.tb14121.x.

DOI:10.1111/j.1432-1033.1988.tb14121.x

PMID:3391163

Abstract

An increased DNA ligase activity is observed in extracts of Bloom's syndrome (BS) fibroblast cell line GM1492. The activity is 2-3-fold higher in this cell line compared to normal human fibroblasts, and 5-20-fold higher than in three other BS cell lines investigated. The DNA ligase activity in GM1492 cells is promoted by a heat-resistant, protease-sensitive factor comigrating with DNA ligases on single-stranded-DNA--cellulose. The factor stimulates DNA ligase I as well as DNA ligase II, and is not identical to the activity-promoting homologous DNA pairing, which is also enhanced in GM1492 cell extracts.

摘要

在布卢姆综合征（BS）成纤维细胞系GM1492的提取物中观察到DNA连接酶活性增加。与正常人成纤维细胞相比，该细胞系中的活性高2至3倍，比所研究的其他三种BS细胞系高5至20倍。GM1492细胞中的DNA连接酶活性由一种耐热、蛋白酶敏感的因子促进，该因子与单链DNA-纤维素上的DNA连接酶一起迁移。该因子刺激DNA连接酶I和DNA连接酶II，并且与在GM1492细胞提取物中也增强的促进活性的同源DNA配对不同。