Gill Amarbir S, Pulsipher Abigail, Sumsion Jorgen S, Oakley Gretchen M, Leclair Laurie W, Howe Heather, Orlandi Richard R, Alt Jeremiah A
Division of Otolaryngology - Head and Neck Surgery, Department of Surgery, University of Utah, Salt Lake City, UT, USA.
School of Medicine, University of Utah, Salt Lake, USA.
J Asthma Allergy. 2021 Apr 21;14:405-413. doi: 10.2147/JAA.S301825. eCollection 2021.
Data regarding the inflammatory profile of patients with asthma and chronic rhinosinusitis (CRS-A) with (CRSwNP-A) and without (CRSsNP-A) nasal polyposis remain limited.
Define and compare systemic transcriptional changes in patients with CRS-A to those with non-asthma-related CRS with (CRSwNP) and without nasal polyposis (CRSsNP).
Thirty-four patients with CRS-A (n=19) and CRS (n=15) were prospectively enrolled into an observational study. Demographic information and subjective and objective disease severity measures were recorded. Multiplex gene expression analysis of mRNA extracted from peripheral blood was performed. A total of 594 genes associated with innate/adaptive immunity were analyzed using NanoString technology. Gene expression ratios were reported for genes that were differentially expressed among these cohorts. Linear regression analysis was used to compare the mRNA transcript copy numbers for each gene with disease severity.
There was no significant difference in age, gender, nasal polyposis, or health-related quality of life measures between the two groups (p>0.05). HLA class II histocompatibility antigen, DRB3-1 beta chain () was significantly upregulated in the peripheral blood of patients with CRSsNP-A compared to CRSsNP, whereas chemokine (C-C motif) ligands 4 () and zinc finger protein helios () were significantly upregulated in CRSwNP-A compared to CRSwNP (p<0.05).
Patients with CRSsNP-A demonstrate a molecular endotype associated with a Th2-dominant inflammatory profile compared to CRSsNP. Patients with CRSwNP-A similarly demonstrate an overrepresentation of genes associated with Th2-driven inflammation compared to patients with CRSwNP.
有关患有哮喘和慢性鼻-鼻窦炎（CRS-A）且伴有（CRSwNP-A）和不伴有（CRSsNP-A）鼻息肉患者的炎症特征的数据仍然有限。
定义并比较CRS-A患者与患有鼻息肉（CRSwNP）和不患有鼻息肉（CRSsNP）的非哮喘相关性CRS患者的全身转录变化。
34例CRS-A患者（n = 19）和CRS患者（n = 15）被前瞻性纳入一项观察性研究。记录人口统计学信息以及主观和客观疾病严重程度指标。对外周血提取的mRNA进行多重基因表达分析。使用NanoString技术分析总共594个与先天性/适应性免疫相关的基因。报告这些队列中差异表达基因的基因表达比率。使用线性回归分析比较每个基因的mRNA转录本拷贝数与疾病严重程度。
两组在年龄、性别、鼻息肉或健康相关生活质量指标方面无显著差异（p>0.05）。与CRSsNP相比，CRSsNP-A患者外周血中HLA II类组织相容性抗原DRB3-1β链（ ）显著上调，而与CRSwNP相比，CRSwNP-A患者中趋化因子（C-C基序）配体4（ ）和锌指蛋白Helios（ ）显著上调（p<0.05）。
与CRSsNP相比，CRSsNP-A患者表现出与Th2主导的炎症特征相关的分子内型。与CRSwNP患者相比，CRSwNP-A患者同样表现出与Th2驱动的炎症相关基因的过度表达。
