Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Jining, Jining, Shandong 272000, P.R. China.
Department of Anesthesiology, The First People's Hospital of Jining, Jining, Shandong 272000, P.R. China.
Mol Med Rep. 2019 May;19(5):3855-3863. doi: 10.3892/mmr.2019.10005. Epub 2019 Mar 5.
Chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the most prevalent chronic diseases. In patients with CRSwNP, the present study performed comprehensive bioinformatics analyses to characterize the transcriptome profiles of mRNAs and long non‑coding RNAs (lncRNAs). A total of 265 differentially expressed lncRNAs and 994 mRNAs were identified. The majority of up‑ and downregulated differentially expressed genes were significantly enriched in the biological process of 'signal transduction'. The most significantly enriched molecular function was 'protein binding' and the most significantly enriched cellular component was 'membrane'. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis led to identification of several significantly enriched pathways [false discovery rate (FDR)<0.05], including 'cytokine‑cytokine receptor interaction' (FDR=3.94x1016) and 'cell adhesion molecules' (CAMs) (FDR=1.28x10‑5). Key differentially expressed lncRNAs were identified, including lncRNA XLOC_010280, which regulates chemokine (C‑C motif) ligand 18 (CCL18) and inflammation, and RP11‑798M19.6, which regulates polypeptide N‑acetylgalactosaminyltransferase 7 (GALNT7) and cell proliferation. Based on the results of reverse transcription‑quantitative polymerase chain reaction, except for CCL8, neural precursor cell expressed developmentally downregulated gene 4‑like and GALNT7, the expression of 3 other selected genes was consistent with the results of integrated analysis. The results of the present study provide a foundation for future investigations into mRNAs and lncRNAs as diagnostic and therapeutic targets in CRSwNP.
伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）是最常见的慢性疾病之一。在 CRSwNP 患者中，本研究进行了全面的生物信息学分析，以描绘 mRNA 和长非编码 RNA（lncRNA）的转录组谱特征。共鉴定出 265 个差异表达的 lncRNA 和 994 个 mRNA。大多数上调和下调的差异表达基因在“信号转导”的生物学过程中显著富集。最显著富集的分子功能是“蛋白质结合”，最显著富集的细胞成分是“膜”。京都基因与基因组百科全书通路富集分析导致鉴定出几个显著富集的通路[错误发现率（FDR）<0.05]，包括“细胞因子-细胞因子受体相互作用”（FDR=3.94x1016）和“细胞黏附分子”（CAMs）（FDR=1.28x10-5）。鉴定出关键差异表达的 lncRNA，包括调节趋化因子（C-C 基序）配体 18（CCL18）和炎症的 lncRNA XLOC_010280，以及调节多肽 N-乙酰半乳糖胺转移酶 7（GALNT7）和细胞增殖的 RP11-798M19.6。基于逆转录-定量聚合酶链反应的结果，除了 CCL8、神经前体细胞表达的发育下调基因 4 样和 GALNT7 外，其他 3 个选定基因的表达与整合分析的结果一致。本研究的结果为进一步研究 mRNAs 和 lncRNAs 作为 CRSwNP 的诊断和治疗靶点提供了基础。
