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在[具体物种名称]中[具体基因名称1]和[具体基因名称2]的分子特征分析 。 （需注意，这里原文信息不完整，实际翻译时应补充完整具体的物种及基因名称等信息使译文更准确清晰）

Molecular characterization of and gene in .

作者信息

Kubra Ghulam, Khan Maryam, Hussain Sidra, Iqbal Tooba, Muhammad Jan, Ali Hina, Gul Alvina, Munir Faiza, Amir Rabia

机构信息

Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad 44000, Pakistan.

National Institute for Lasers and Optronics (NILOP), Lehtrar Road, Islamabad 44000, Pakistan.

出版信息

Saudi J Biol Sci. 2021 Apr;28(4):2301-2315. doi: 10.1016/j.sjbs.2021.01.024. Epub 2021 Jan 23.

DOI:10.1016/j.sjbs.2021.01.024
PMID:33911945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8071922/
Abstract

(peanut) is a potential source of bioactive compounds including flavonols and proanthocyanidins, which have gained particular interest of metabolic engineering owing to their significance in the growth, development and defense responses in plants. To gain insight of proanthocyanidins and flavonols production in , and enzymes responsible for their production, have been structurally, transcriptionally and functionally characterized. Structural and functional analysis of putative protein sequence of indicated two functional motifs 2OG-FeII_Oxy and DIOX_N while six functional motifs belonging to the families of NAD-dependent dehydratase, 3, and NmrA-like family were observed in case of Promoter sequence analysis unraveled several promoter elements related to the development regulation, environmental stress responses and hormonal signaling. Furthermore, the expression analysis of and and accumulation pattern analysis of proanthocyanidins and flavonols in three selected cultivars of under saline environment confirmed their role against salinity in genotype-dependent and stress level-dependent manner. Correlation studies revealed that AhFLS and AhLAR expression is not directly dependent on the antioxidant enzymes activity, biochemical and growth parameters but higher Pearson r value depicted some level of dependency. This detailed study of AhLAR and AhFLS can assist in the metabolic engineering of flavonoid biosynthetic pathway to produce stress tolerant varieties and production of proanthocyanidins and flavonols at an industrial scale.

摘要

（花生）是生物活性化合物的潜在来源，包括黄酮醇和原花青素，由于它们在植物生长、发育和防御反应中的重要性，已引起代谢工程的特别关注。为了深入了解花生中原花青素和黄酮醇的产生以及负责其产生的酶，对相关酶进行了结构、转录和功能表征。对假定蛋白质序列的结构和功能分析表明，存在两个功能基序2OG-FeII_Oxy和DIOX_N，而在另一种酶的情况下，观察到属于NAD依赖性脱水酶、3和NmrA样家族的六个功能基序。启动子序列分析揭示了几个与发育调控、环境应激反应和激素信号传导相关的启动子元件。此外，在盐环境下对三个选定花生品种中相关酶的表达分析以及原花青素和黄酮醇的积累模式分析，证实了它们在基因型依赖性和胁迫水平依赖性方式下对盐度的作用。相关性研究表明，AhFLS和AhLAR的表达并不直接依赖于抗氧化酶活性、生化和生长参数，但较高的皮尔逊r值表明存在一定程度的依赖性。对AhLAR和AhFLS的这项详细研究有助于黄酮类生物合成途径的代谢工程，以培育耐胁迫品种，并实现原花青素和黄酮醇的工业规模生产。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/3427efe70d24/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/da34f26f4a98/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/f19100751e48/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/c63df11eaaf7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/66fe3019017d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/91945e466b52/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/f9761ea67e03/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/383cfbc2ccd7/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/4d86d64199d9/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/e2432a16b3f5/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/b1e3afd6ac29/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/3427efe70d24/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/da34f26f4a98/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/f19100751e48/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/c63df11eaaf7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/66fe3019017d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/91945e466b52/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/f9761ea67e03/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/383cfbc2ccd7/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/4d86d64199d9/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/e2432a16b3f5/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/b1e3afd6ac29/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef38/8071922/3427efe70d24/gr11.jpg

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