融合后细胞密度对杂交瘤细胞建立的影响。
The effect of postfusion cell density on establishment of hybridomas.
作者信息
Kontseková E, Novák M, Kontsek P, Borecký L, Lesso J
机构信息
Department of Microbiology, Virology and Immunology, Comenius University, Bratislava.
出版信息
Folia Biol (Praha). 1988;34(1):18-22.
The effect of postfusion cell density on establishment of hybridomas in primary microculture was studied. Following fusion of BALB/c spleen and NS0 myeloma cells in the ratio of 5:1 we demonstrated that seeding the cells at a concentration of about 20-25 X 10(4) spleen cells per well resulted in maximal number of wells containing only one growing hybridoma clone (40.1-43.7% plated wells). Importance of generation of microculture with one clone for the effectiveness of preparation of mAb-secreting hybridomas is discussed.
研究了融合后细胞密度对原代微量培养中杂交瘤细胞建立的影响。以5:1的比例将BALB/c脾细胞与NS0骨髓瘤细胞融合后，我们发现，每孔以约20 - 25×10⁴个脾细胞的浓度接种细胞，会使仅含有一个生长杂交瘤克隆的孔数量达到最多（接种孔的40.1 - 43.7%）。文中讨论了产生单克隆微量培养对于制备分泌单克隆抗体杂交瘤细胞有效性的重要性。
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