与九英里菌株发生交叉反应的针对伯纳特立克次体的单克隆抗体。
Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile.
作者信息
Sekeyová Z, Kovácová E, Kazár J, Toman R, Olvecká S
机构信息
Department of Rickettsiae, Slovak Academy of Sciences, Bratislava.
出版信息
Clin Diagn Lab Immunol. 1995 Sep;2(5):531-4. doi: 10.1128/cdli.2.5.531-534.1995.
Abstract
Results are presented to show the binding properties of five monoclonal antibodies directed to Coxiella burnetii Priscilla with cross-reactions to the Nine Mile strain. The monoclonal antibodies preferentially recognize phase I epitopes by ELISA and recognize phase II epitopes by immunoblotting but do not allow differentiation between so-called chronic and acute strains of C. burnetii. The only difference in reactivity was in the staining pattern revealed after reactions with lipopolysaccharide I antigens.
摘要
结果表明，5种针对伯氏考克斯氏体普里西拉菌株且与九英里菌株有交叉反应的单克隆抗体的结合特性。这些单克隆抗体通过酶联免疫吸附测定（ELISA）优先识别I相表位，通过免疫印迹识别II相表位，但无法区分所谓的伯氏考克斯氏体慢性菌株和急性菌株。反应性的唯一差异在于与脂多糖I抗原反应后显示的染色模式。
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