Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, USP - Universidade de São Paulo, São Paulo, SP, Brazil.
Department of Orthodontic, School of Dentistry of Ribeirao Preto, USP - Universidade de São Paulo, Ribeirao Preto, SP, Brazil.
Braz Dent J. 2021 Jan-Feb;32(1):98-103. doi: 10.1590/0103-6440202103527.
Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) have the ability to increase vascular proliferation and permeability. The aim of this study was to quantify the release of two diffusible angiogenic growth factors (VEGF and FGF-2) after rapid maxillary expansion (RME). Thirty animals were randomly assigned to two groups. Control group (5 rats - intact suture) and Experimental groups (25 rats with RME) which were evaluated in different periods of treatment. Five animals were euthanized in different periods of healing at 0, 1, 2, 3, 5 and 7 days after RME. RT-PCR was used to evaluate the gene expression of angiogenic growth factors released on different periods of study. Data were submitted to statistical analysis using ANOVA followed by Tukey test and significance was assumed at a=0.05. RT-PCR showed that mRNAs of VEGF and FGF-2 were expressed in intact palatal suture tissue. mRNAs of VEGF and FGF-2 was upregulated in early periods (24 h) after RME (p<0.001 and p<0.01, respectively). The molecular levels of VEGF never returned to its original baseline values, and FGF-2 expression decreased up to day 5 (p<0.001) and suddenly increased at day 7, returning to its original level. RME increased VEGF secretion, but decreased FGF-2 secretion when compared to intact tissue. The results showed that these angiogenic growth factors are released and regulated in the palatal suture tissue after RME and could make an important contribution to the knowledge of overall reparative response of the suture tissue during the bone remodeling process.
血管内皮生长因子 (VEGF) 和碱性成纤维细胞生长因子 (FGF-2) 具有增加血管增殖和通透性的能力。本研究的目的是量化快速上颌扩张 (RME) 后两种可扩散血管生成生长因子 (VEGF 和 FGF-2) 的释放。30 只动物被随机分为两组。对照组 (5 只大鼠 - 完整的缝合线) 和实验组 (25 只大鼠进行 RME)，分别在不同的治疗期进行评估。在 RME 后 0、1、2、3、5 和 7 天的不同愈合期处死 5 只动物。RT-PCR 用于评估在不同研究期间释放的血管生成生长因子的基因表达。使用方差分析 (ANOVA) 对数据进行统计分析，然后进行 Tukey 检验，假设显著性水平为 a=0.05。RT-PCR 显示 VEGF 和 FGF-2 的 mRNA 在完整的腭缝合组织中表达。RME 后早期 (24 小时) VEGF 和 FGF-2 的 mRNA 表达上调 (p<0.001 和 p<0.01)。VEGF 的分子水平从未恢复到原始基线值，而 FGF-2 的表达在第 5 天下降 (p<0.001)，并在第 7 天突然增加，恢复到原始水平。与完整组织相比，RME 增加了 VEGF 的分泌，但减少了 FGF-2 的分泌。结果表明，这些血管生成生长因子在 RME 后在腭缝合组织中释放并受到调节，这可能对缝合组织在骨重塑过程中的整体修复反应的知识做出重要贡献。
