Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice, Czech Republic.
Potato Research Institute Havlíčkův Brod, Dobrovského 2366, Havlíčkův Brod, 580 01, Czech Republic.
Virus Genes. 2021 Jun;57(3):302-305. doi: 10.1007/s11262-021-01838-9. Epub 2021 Apr 29.
Two novel dsDNA bacteriophages named Pectobacterium virus CB251 (PcCB251) and Pectobacterium virus CB7V (PcCB7V) targeting plant pathogen Pectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. The phage is classified in genus Berlinvirus, family Autographiviridae. The PcCB7V phage has a circular dsDNA genome of 146,054 bp with G+C content of 50.4% and contains 269 predicted protein genes on both strands and 13 tRNA genes. The PcCB7V phage can be classified in genus Certrevirus, subfamily Vequintavirinae. Both novel bacteriophages have narrow host ranges, but they extend the list of candidates for phage-based control of pectolytic bacteria causing soft rot disease of potato.
两种新型 dsDNA 噬菌体,命名为 Pectobacterium virus CB251(PcCB251)和 Pectobacterium virus CB7V(PcCB7V),已被分离并测序,它们的宿主是植物病原体 Pectobacterium parmentieri。PcCB251 基因组由 40557 bp 组成,GC 含量为 48.6%,单链上包含 47 个预测基因。噬菌体被归类为 Berlinvirus 属,Autographiviridae 科。PcCB7V 噬菌体具有一个圆形双链 DNA 基因组,大小为 146054 bp,GC 含量为 50.4%,双链上包含 269 个预测蛋白基因和 13 个 tRNA 基因。PcCB7V 噬菌体可归类为 Certrevirus 属,Vequintavirinae 亚科。这两种新型噬菌体的宿主范围都很窄,但它们扩大了基于噬菌体控制引起马铃薯软腐病的果胶分解细菌的候选名单。