Kabanova Anastasia, Shneider Mikhail, Bugaeva Eugenia, Ha Vo Thi Ngoc, Miroshnikov Kirill, Korzhenkov Aleksei, Kulikov Eugene, Toschakov Stepan, Ignatov Alexander, Miroshnikov Konstantin
M.M. Shemyakin-Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Research Center "PhytoEngineering" Ltd., Rogachevo, Moscow Region, Russia.
Arch Virol. 2018 Jun;163(6):1691-1694. doi: 10.1007/s00705-018-3766-1. Epub 2018 Feb 8.
Bacteriophage vB_PpaP_PP74 (PP74) is a novel virulent phage that infects members of the species Pectobacterium parmentieri, a newly established species of soft-rot-causing bacteria in the family Pectobacteriaceae, derived from potato-specific Pectobacterium wasabiae. vB_PpaP_PP74 was identified as a member of the family Podoviridae by transmission electron microscopy. The phage has a 39,790-bp dsDNA genome containing 50 open reading frames (ORFs). Because of the absence of genes encoding toxins or lysogeny factors, PP74 may be considered a candidate phage for pathogen biocontrol applications. The genome layout is similar to genomes of T7-like phages within the subfamily Autographivirinae, and therefore, functions can be attributed to most of ORFs. However, the closest nucleotide sequence homologs of phage PP74 are unclassified Escherichia phages. Based on phylogenetic analysis, vB_PpaP_PP74 is a sensu lato T7-like phage, but it forms a distant subgenus group together with homologous enterobacterial phages.
噬菌体vB_PpaP_PP74（PP74）是一种新型烈性噬菌体，可感染胡萝卜软腐果胶杆菌，该菌是果胶杆菌科中一个新建立的引起软腐病的细菌物种，源自马铃薯专性致病的山葵果胶杆菌。通过透射电子显微镜鉴定，vB_PpaP_PP74属于短尾噬菌体科。该噬菌体具有一个39790 bp的双链DNA基因组，包含50个开放阅读框（ORF）。由于缺乏编码毒素或溶原性因子的基因，PP74可被视为病原体生物防治应用的候选噬菌体。其基因组布局与自复制病毒亚科内T7样噬菌体的基因组相似，因此，大多数开放阅读框的功能可以确定。然而，噬菌体PP74最接近的核苷酸序列同源物是未分类的大肠杆菌噬菌体。基于系统发育分析，vB_PpaP_PP74是一种广义的T7样噬菌体，但它与同源肠道细菌噬菌体一起形成了一个远缘亚属群。
