Laboratory of Cell Biology and Microbiology, Academic Unit of Biological Sciences, Autonomous University of Zacatecas, Zacatecas, México.
Laboratory of Immunology and Virology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolás de los Garza, México.
Parasite Immunol. 2021 Aug;43(8):e12840. doi: 10.1111/pim.12840. Epub 2021 May 10.
The immune response during T spiralis infection is characterized by an increase in eosinophils and mast cells, as well as Th2 cytokine production, such as interleukin (IL)-4, IL-10 and IL-13, promoting T spiralis expulsion from the host. However, this response damages the host, favouring the parasite survival. In the search for new pharmacological strategies that protect against T spiralis infection, a recent study showed that treatment with resiniferatoxin (RTX) modulates the Th1 cytokines production, reducing muscle parasite burden.
To evaluate the effect of RTX treatment on the Th2 cytokines production, the number of eosinophils, mast cells and the intestinal expulsion of T spiralis.
Serum levels of IL-4, IL-10 and IL-13 were quantified by ELISA; the number of eosinophils, mast cells and the adult worms of T spiralis in the small intestine was quantified.
RTX treatment increased serum levels of IL-4, IL-10 and IL-13, and it decreases intestinal eosinophilia, however, favours the mastocytosis, promoting T spiralis intestinal expulsion.
These findings suggest that RTX is capable to modulate the Th2 immune response, promoting T spiralis expulsion, which contributes to the defence against T spiralis infection, placing the RTX as a potential immunomodulatory drug.
旋毛虫感染期间的免疫反应表现为嗜酸性粒细胞和肥大细胞增多，以及 Th2 细胞因子（如白细胞介素-4、白细胞介素-10 和白细胞介素-13）的产生增加，从而促进旋毛虫从宿主中排出。然而，这种反应会损害宿主，有利于寄生虫的生存。在寻找新的药物治疗策略以预防旋毛虫感染的过程中，最近的一项研究表明，使用瑞香素（RTX）治疗可调节 Th1 细胞因子的产生，减少肌肉寄生虫负担。
评估 RTX 治疗对 Th2 细胞因子产生、嗜酸性粒细胞、肥大细胞数量以及旋毛虫肠道排出的影响。
通过 ELISA 定量检测血清中白细胞介素-4、白细胞介素-10 和白细胞介素-13 的水平；定量检测小肠中的嗜酸性粒细胞、肥大细胞和旋毛虫成虫数量。
RTX 治疗增加了血清中白细胞介素-4、白细胞介素-10 和白细胞介素-13 的水平，减少了肠道嗜酸性粒细胞浸润，但促进了肥大细胞增生，促进了旋毛虫的肠道排出。
这些发现表明，RTX 能够调节 Th2 免疫反应，促进旋毛虫的排出，这有助于抵抗旋毛虫感染，将 RTX 作为一种潜在的免疫调节药物。
