Muñoz-Carrillo J L, Contreras-Cordero J F, Muñoz-López J L, Maldonado-Tapia C H, Muñoz-Escobedo J J, Moreno-García M A
Laboratory of Cell Biology and Microbiology, Academic Unit of Biological Sciences, Autonomous University of Zacatecas, Zacatecas, México.
Laboratory of Immunology and Virology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolás de los Garza, Nuevo León, México.
Parasite Immunol. 2017 Sep;39(9). doi: 10.1111/pim.12448. Epub 2017 Jun 27.
In the early stage of the intestinal phase of Trichinella spiralis infection, the host triggers a Th1-type immune response with the aim of eliminating the parasite. However, this response damages the host which favours the survival of the parasite. In the search for novel pharmacological strategies that inhibit the Th1 immune response and assist the host against T. spiralis infection, a recent study showed that resiniferatoxin had anti-inflammatory activity contributed to the host in T. spiralis infection. In this study, we evaluated whether RTX modulates the host immune response through the inhibition of Th1 cytokines in the intestinal phase. In addition, it was determined whether the treatment with RTX affects the infectivity of T. spiralis-L1 and the development of the T. spiralis life cycle. Our results show that RTX decreased serum levels of IL-12, INF-γ, IL-1β, TNF-α and parasite burden on muscle tissue. It was observed that T. spiralis-L1 treated with RTX decreased their infectivity affecting the development of the T. spiralis life cycle in mouse. These results demonstrate that RTX is able to inhibit the production of Th1 cytokines, contributing to the defence against T. spiralis, which places it as a potential drug modulator of the immune response.
在旋毛虫感染肠道期的早期，宿主触发Th1型免疫反应以消除寄生虫。然而，这种反应会损害宿主，从而有利于寄生虫的存活。在寻找抑制Th1免疫反应并帮助宿主抵抗旋毛虫感染的新型药理学策略的过程中，最近一项研究表明，树脂毒素具有抗炎活性，对旋毛虫感染的宿主有益。在本研究中，我们评估了树脂毒素是否通过抑制肠道期的Th1细胞因子来调节宿主免疫反应。此外，还确定了树脂毒素处理是否会影响旋毛虫L1的感染力以及旋毛虫生命周期的发展。我们的结果表明，树脂毒素降低了血清中白细胞介素-12、干扰素-γ、白细胞介素-1β、肿瘤坏死因子-α的水平以及肌肉组织中的寄生虫负荷。观察到用树脂毒素处理的旋毛虫L1降低了它们的感染力，影响了小鼠体内旋毛虫生命周期的发展。这些结果表明，树脂毒素能够抑制Th1细胞因子的产生，有助于抵抗旋毛虫，这使其成为一种潜在的免疫反应药物调节剂。
