Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Int J Mol Sci. 2021 Apr 1;22(7):3665. doi: 10.3390/ijms22073665.
The aim of this study was to develop magnetic molecularly imprinted nano-conjugate sorbent for effective dispersive solid phase extraction of antazoline (ANT) and its metabolite, hydroxyantazoline (ANT-OH) in analytical method employing liquid chromatography coupled with mass spectrometry method. The core-shell material was characterized in terms of adsorption properties, morphology and structure. The heterogeneous population of adsorption sites towards ANT-OH was characterized by two K and two B values: K (1) = 0.319 µg L and B (1) = 0.240 μg g, and K (2) = 34.6 µg L and B (2) = 5.82 μg g. The elemental composition of magnetic sorbent was as follows: 17.55, 37.33, 9.14, 34.94 wt% for Si, C, Fe and O, respectively. The extraction protocol was optimized, and the obtained results were explained using theoretical analysis. Finally, the analytical method was validated prior to application to pharmacokinetic study in which the ANT was administrated intravenously to three healthy volunteers. The results prove that the novel sorbent could be useful in extraction of ANT and ANT-OH from human plasma and that the analytical strategy could be a versatile tool to explain a potential and pharmacological activity of ANT and ANT-OH.
本研究旨在开发用于有效分散固相萃取的磁性分子印迹纳米复合吸附剂，用于分析方法，采用液相色谱 - 质谱联用方法。核壳材料的吸附性能、形态和结构进行了表征。通过两个 K 和两个 B 值对 ANT-OH 的异质吸附位点进行了表征：K(1)=0.319μg/L 和 B(1)=0.240μg/g，以及 K(2)=34.6μg/L 和 B(2)=5.82μg/g。磁性吸附剂的元素组成为：17.55、37.33、9.14、34.94wt%分别为 Si、C、Fe 和 O。优化了萃取方案，并使用理论分析解释了获得的结果。最后，在应用于健康志愿者静脉注射 ANT 的药代动力学研究之前，对分析方法进行了验证。结果证明，新型吸附剂可用于从人血浆中提取 ANT 和 ANT-OH，分析策略可能是解释 ANT 和 ANT-OH 的潜在和药理学活性的通用工具。
