Laboratory of Epidemiology, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Jilin 130122, China.
Viruses. 2021 Apr 1;13(4):606. doi: 10.3390/v13040606.
Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressing the capsid protein was constructed using the baculovirus expression vector system (BEVS). A subunit vaccine was developed based on the capsid protein with appropriate adjuvant. Then, a field trial was carried out in two districts in order to evaluate the efficiency of the subunit vaccine. The field trial indicated that in total, only 1.8% of the minks developed typical diarrhea in the vaccinated group compared with 74.5% in the control group. The vaccination could significantly reduce the infection rate of MiCV among the mink herds and could restrain the virus' shedding from feces. Furthermore, the vaccinated group had a higher average litter size in the following year compared to the control group. Collectively, the results indicated that the subunit vaccine based on the capsid protein can provide reliable protection against MiCV infection.
水貂顽固性腹泻是一种在我国许多水貂养殖场发生的季节性疾病。水貂圆环病毒（MiCV）已被认为是该病的病原体。本研究旨在开发一种针对水貂顽固性腹泻的亚单位疫苗。使用杆状病毒表达载体系统（BEVS）构建了表达衣壳蛋白的重组杆状病毒株。基于衣壳蛋白和适当的佐剂开发了亚单位疫苗。然后，在两个区进行了现场试验，以评估亚单位疫苗的效果。现场试验表明，在接种组中，只有 1.8%的水貂出现典型腹泻，而对照组中则有 74.5%的水貂出现典型腹泻。接种疫苗可显著降低水貂群中 MiCV 的感染率，并抑制病毒从粪便中排出。此外，接种组在下一年的平均窝产仔数高于对照组。总的来说，这些结果表明，基于衣壳蛋白的亚单位疫苗可以为 MiCV 感染提供可靠的保护。
