Vidal J
Department of Microbiology and Immunology, Albert B. Chandler Medical Center, University of Kentucky, Lexington.
Immunobiology. 1988 Mar;176(4-5):329-40. doi: 10.1016/S0171-2985(88)80017-2.
Immunization of (DBA/2) mice with sheep erythrocytes (1 X 10(8) red cells, once weekly for 2 months) elicited anti-sheep erythrocyte antibodies, a part of which combined with ssDNA. By contrast, immunization with rat (Fisher) erythrocytes (1 X 10(8) red cells, once weekly for 2 weeks) did not elicit antibodies cross-reactive with ssDNA. The antibody response (IgM and IgG) to sheep erythrocytes rose sharply and subsequently tapered off (usually within the first 2 weeks). The level of IgG antibodies cross-reactive with ssDNA increased and, after ca. 1 month, decreased. No increase in anti-trinitrophenyl antibodies was detected. These results suggest the existence of a homeostatic mechanism. The anti-ssDNA antibodies bound to sheep erythrocytes, ssDNA and, marginally, to trinitrophenyl-gelatin; they did not bind to poly-D-glutamic acid, rat erythrocytes or mouse erythrocytes. Treatment of sheep red blood cells with neuraminidase, proteinase K, trypsin, or DNase did not alter the erythrocytes' capacity to bind the anti-ssDNA antibodies; solubilization of the erythrocytes with Triton X-100 abolished the binding. Neither a methanol:chloroform (1:1) extract (which contains the erythrocyte phospholipids) nor the residue (left after the extraction) bound anti-ssDNA antibodies. The determinant mediating the binding could be conformational.
用绵羊红细胞（1×10⁸个红细胞，每周一次，共2个月）对（DBA/2）小鼠进行免疫接种可引发抗绵羊红细胞抗体，其中一部分与单链DNA结合。相比之下，用大鼠（Fisher）红细胞（1×10⁸个红细胞，每周一次，共2周）进行免疫接种未引发与单链DNA交叉反应的抗体。对绵羊红细胞的抗体反应（IgM和IgG）急剧上升，随后逐渐下降（通常在最初2周内）。与单链DNA交叉反应的IgG抗体水平先升高，约1个月后下降。未检测到抗三硝基苯基抗体增加。这些结果表明存在一种稳态机制。抗单链DNA抗体与绵羊红细胞、单链DNA以及少量与三硝基苯基明胶结合；它们不与聚-D-谷氨酸、大鼠红细胞或小鼠红细胞结合。用神经氨酸酶、蛋白酶K、胰蛋白酶或脱氧核糖核酸酶处理绵羊红细胞不会改变红细胞结合抗单链DNA抗体的能力；用Triton X-100使红细胞溶解会消除这种结合。甲醇：氯仿（1:1）提取物（含有红细胞磷脂）及其提取物后的残留物均不结合抗单链DNA抗体。介导这种结合的决定簇可能是构象性的。
