疟原虫多蛋白转运器2（MC-2TM）与疟原虫毛氏小管的转运及关联

Trafficking and Association of MC-2TM with the Maurer's Clefts.

作者信息

Yadavalli Raghavendra, Peterson John W, Drazba Judith A, Sam-Yellowe Tobili Y

机构信息

Department of Biological, Geological, and Environmental Sciences, Cleveland State University, Cleveland, OH 44115, USA.

Imaging Core Facility, The Cleveland Clinic, Cleveland, OH 44195, USA.

出版信息

Pathogens. 2021 Apr 5;10(4):431. doi: 10.3390/pathogens10040431.

In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive with PfMC-2TM. PfMC-2TM is sensitive to BFA treatment and permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associated with the MC and infected erythrocyte membrane and was resistant to extraction with alkaline sodium carbonate, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs.

在本研究中，我们调查了内源性疟原虫膜内复合物-2跨膜蛋白（PfMC-2TM）在（3D7）感染红细胞中的阶段特异性表达、运输、溶解性和拓扑结构。在用布雷菲德菌素A（BFA）处理寄生虫后，使用与PfMC-2TM反应的抗体通过免疫荧光评估PfMC-2TM的运输。PfMC-2TM对BFA处理敏感，用链球菌溶血素O（SLO）和皂角苷对感染红细胞进行通透处理表明，PfMC-2TM的N端和C端暴露于红细胞胞质中，而蛋白质的中央部分在膜内复合物（MC）膜中受到保护。PfMC-2TM早在入侵后4小时（hpi）就开始表达，与REX-1紧密共定位，并运输到红细胞膜，溶解性没有变化。PfMC-2TM与MC和感染红细胞膜相关，并且对碳酸钠提取具有抗性，提示其与MC和红细胞膜存在蛋白质-脂质相互作用。PfMC-2TM是新生MC的另一个标志物。