Denham S, Hall J G
Experimental Unit, Institute of Cancer Research, Sutton, Surrey, U.K.
Immunology. 1988 Jun;64(2):341-4.
Efferent lymph from the popliteal or prefemoral lymph nodes was collected from unanaesthetized sheep before and after the injection of insoluble Be(OH)2 into the subcuticulum that was drained by the nodes. Sterile, cell-free plasma from lymph collected during the 48 hr after the injections, induced vigorous lympho-proliferative responses in naive lymph nodes after s.c. injection into autochthonous or allogeneic recipients. The same lymph had an adjuvant action when injected i.p. into both nude and euthymic rats. Many samples of such lymph were screened for biological activity in rats by noting the increase in the weight of the regional popliteal nodes that occurred after the test sample had been injected into the footpad. All the active samples were shown, by atomic absorbance spectroscopy, to contain beryllium, and we concluded that this metal, in the form of soluble Be-protein adducts, was probably the prime mover in all the observed responses.
在将不溶性氢氧化铍注射到由腘窝或股前淋巴结引流的皮下组织之前和之后，从未麻醉的绵羊身上收集来自这些淋巴结的输出淋巴。注射后48小时内收集的淋巴中的无菌、无细胞血浆，在皮下注射到自体或异体受体的幼稚淋巴结后，可诱导强烈的淋巴细胞增殖反应。当腹腔注射到裸鼠和有胸腺大鼠体内时，相同的淋巴具有佐剂作用。通过记录将测试样品注射到足垫后区域腘窝淋巴结重量的增加，对许多这样的淋巴样品在大鼠中进行生物活性筛选。通过原子吸收光谱法显示，所有活性样品都含有铍，我们得出结论，这种以可溶性铍 - 蛋白质加合物形式存在的金属，可能是所有观察到的反应中的主要推动者。
