Lee Soohong, Vu Nguyen-Trung, Oh Eom-Ji, Rahimi-Midani Aryan, Thi Thuong-Nguyen, Song Yu-Rim, Hwang In-Sun, Choi Tae-Jin, Oh Chang-Sik
Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Korea.
Department of Microbiology, Pukyong National University, Busan 48513, Korea.
Microorganisms. 2021 Apr 8;9(4):779. doi: 10.3390/microorganisms9040779.
has recently emerged as a widely infective and destructive pathogen causing soft-rot disease in various vegetables. Bacteriophage phiPccP-1 isolated from Pyeongchang, South Korea, showed lytic activity against Pco14 and two other species. The transmission electron microscopy and genome phylograms revealed that phiPccP-1 belongs to the genus, subfamily of the family. Genome comparison showed that its 40,487 bp double-stranded DNA genome shares significant similarity with phage DU_PP_II with the identity reaching 98% of the genome. The phiPccP-1 application significantly inhibited the development of soft-rot disease in the mature leaves of the harvested Kimchi cabbage up to 48 h after Pco14 inoculation compared to the untreated leaves, suggesting that phiPccP-1 can protect Kimchi cabbage from soft-rot disease after harvest. Remarkably, bioassays with phiPccP-1 in Kimchi cabbage seedlings grown in the growth chamber successfully demonstrated its prophylactic and therapeutic potential in the control of bacterial soft-rot disease in Kimchi cabbage. These results indicate that bacteriophage phiPccP-1 can be used as a potential biological agent for controlling soft rot disease in Kimchi cabbage.
最近已成为一种广泛感染且具有破坏性的病原体，可导致各种蔬菜发生软腐病。从韩国平昌分离出的噬菌体phiPccP-1对Pco14和其他两个物种表现出裂解活性。透射电子显微镜和基因组系统发育图显示，phiPccP-1属于该科的属、亚科。基因组比较表明，其40487 bp的双链DNA基因组与噬菌体DU_PP_II具有显著相似性，基因组同一性达到98%。与未处理的叶片相比，phiPccP-1的应用在Pco14接种后长达48小时内显著抑制了收获的泡菜白菜成熟叶片中软腐病的发展，这表明phiPccP-1可以在收获后保护泡菜白菜免受软腐病侵害。值得注意的是，在生长室中种植的泡菜白菜幼苗中用phiPccP-1进行的生物测定成功证明了其在控制泡菜白菜细菌性软腐病方面的预防和治疗潜力。这些结果表明，噬菌体phiPccP-1可作为控制泡菜白菜软腐病的潜在生物制剂。
