Jee Samnyu, Choi Jang-Gyu, Lee Young-Gyu, Kwon Min, Hwang Ingyu, Heu Sunggi
Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea.
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
Plant Pathol J. 2020 Aug 1;36(4):346-354. doi: 10.5423/PPJ.OA.09.2019.0235.
, which causes soft rot disease, is divided into 18 species based on the current classification. A total of 225 strains were isolated from 10 main cultivation regions of potato (), napa cabbage ( subsp. ), and radish () in South Korea; 202 isolates (90%) were from potato, 18 from napa cabbage, and five from radish. Strains were identified using the Biolog test and phylogenetic analysis. The pathogenicity and swimming motility were tested at four different temperatures. Pectolytic activity and plant cell-wall degrading enzyme (PCWDE) activity were evaluated for six species ( subsp. , Pcc; , Pod; , Pbr; , Pve; , Ppo; , Ppa). Pod, Pcc, Pbr, and Pve were the most prevalent species. Although is a widespread pathogen in other countries, it was not found here. This is the first report of Ppo, Ppa, and Pve in South Korea. species showed stronger activity at 28°C and 32°C than at 24°C, and showed weak activity at 37°C. Pectolytic activity decreased with increasing temperature. Activity of pectate lyase was not significantly affected by temperature Activity of protease, cellulase, and polygalacturonase decreased with increasing temperature. The inability of isolated to soften host tissues at 37°C may be a consequence of decreased motility and PCWDE activity. These data suggest that future increases in temperature as a result of climate change may affect the population dynamics of .
引起软腐病的[病原菌名称未给出]，根据目前的分类可分为18个种。从韩国马铃薯（[马铃薯学名未给出]）、大白菜（[大白菜亚种学名未给出]亚种）和萝卜（[萝卜学名未给出]）的10个主要种植区共分离出225株菌株；202株（90%）来自马铃薯，18株来自大白菜，5株来自萝卜。使用Biolog测试和系统发育分析对菌株进行鉴定。在四个不同温度下测试了致病性和游动性。对六个种（[六个种的学名未给出]亚种、Pcc；[种名未给出]、Pod；[种名未给出]、Pbr；[种名未给出]、Pve；[种名未给出]、Ppo；[种名未给出]、Ppa）的果胶分解活性和植物细胞壁降解酶（PCWDE）活性进行了评估。Pod、Pcc、Pbr和Pve是最常见的种。尽管[病原菌名称未给出]在其他国家是一种广泛存在的病原菌，但在这里未发现。这是韩国首次报道Ppo、Ppa和Pve。[病原菌名称未给出]种在28°C和32°C时的活性比在24°C时更强，在37°C时活性较弱。果胶分解活性随温度升高而降低。果胶酸裂解酶的活性不受温度显著影响。蛋白酶、纤维素酶和多聚半乳糖醛酸酶的活性随温度升高而降低。分离出的[病原菌名称未给出]在37°C时无法软化宿主组织可能是游动性和PCWDE活性降低的结果。这些数据表明，未来气候变化导致的温度升高可能会影响[病原菌名称未给出]的种群动态。
