Kilic Isabel, Stehr Manuel, Hennies Mark, Metges Cornelia C, Franzenburg Sören, Falker-Gieske Clemens, Tetens Jens, Daş Gürbüz
Department of Animal Sciences, University of Göttingen, 37077 Göttingen, Germany.
Institute of Nutritional Physiology 'Oskar Kellner', Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany.
Animals (Basel). 2021 Apr 2;11(4):1002. doi: 10.3390/ani11041002.
Here, we describe the first transcriptomic investigation of the peripheral blood of chickens exposed to and infections. We investigated differentially expressed gene (DEG) patterns in two chicken genotypes with either a higher (Lohmann Brown Plus, LB) or lower (Lohmann Dual, LD) laying performance level. The hens were experimentally coinfected with and , and their worm burdens and infection parameters were determined six weeks post infection. Based on most representative infection parameters, the hens were clustered into lower- and higher-infection intensity classes. We identified a total of 78 DEGs contributing to infection-related phenotypic variation in the two genotypes. Our data showed significant upregulation of () in LD hens, making it a promising candidate for tolerance to ascarid infections in chickens. Gene ontology analysis revealed higher transcriptome activity related to biological processes such as "response to external stimulus" in LB hens, implying a higher stress response in this genotype. In contrast, LD hens showed higher transcriptomic expression of genes related to ontology classes that are possibly associated with a higher tolerance to infections. These findings may help explain why lower-performing genotypes (i.e., LD) are less sensitive to infections in terms of maintaining their performance.
在此，我们描述了对暴露于和感染的鸡外周血进行的首次转录组学研究。我们调查了两种产蛋性能水平较高（罗曼褐 Plus，LB）或较低（罗曼双交，LD）的鸡基因型中差异表达基因（DEG）模式。母鸡通过实验同时感染和，感染六周后测定其蠕虫负荷和感染参数。基于最具代表性的感染参数，母鸡被分为低感染强度组和高感染强度组。我们共鉴定出78个导致两种基因型感染相关表型变异的差异表达基因。我们的数据显示，LD母鸡中的显著上调，使其成为鸡对蛔虫感染耐受性的一个有前景的候选基因。基因本体分析显示，LB母鸡中与“对外部刺激的反应”等生物过程相关的转录组活性较高，这意味着该基因型的应激反应较高。相比之下，LD母鸡显示出与本体类别相关的基因转录组表达较高，这些本体类别可能与对感染的更高耐受性相关。这些发现可能有助于解释为什么性能较低的基因型（即LD）在维持其性能方面对感染不太敏感。
