Sfacteria Alessandra, Napoli Ettore, Rifici Claudia, Commisso Daria, Giambrone Giada, Mazzullo Giuseppe, Marino Gabriele
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.
Animals (Basel). 2021 Apr 21;11(5):1189. doi: 10.3390/ani11051189.
Inflammatory cells have a role in tumor progression and have prognostic and therapeutic potential. The immunohistochemical expression for Mast Cell Tryptase, Macrophage Marker, CD79a, IgA, IgM and IgG on 43 cases of canine mammary gland lesions was analyzed. In hyperplasia, a few B cells (BCs) and Tumor-Associated Macrophages (TAMs) were observed, while the number of Tumor-Associated Mast Cells (TAMCs) was the highest. In the peritumoral stroma of malignant lesions, low number of TAMCs and a high number of TAMAs and BCs were present. Immune cells of each type were always lower in the intratumoral than peritumoral stroma. Positivity to CD79a was also detected in the epithelial cells of simple and micropapillay carcinomas. Immunoglobulin reactivity was mainly located in the epithelial cells where an intense positivity to IgA and IgG and a weak positivity for IgM were detectable. On the basis of our preliminary results and literature data, we suggest that such cells and molecules could be directly involved in the biology of canine mammary gland tumors. In breast cancer, stromal inflammatory cells and cancer derived immunoglobulins have been correlated with the progression, malignancy and poor prognosis of the tumor. The results herein reported show that the dog's mammary gland epithelium also expresses immunoglobulins, and they mostly show a direct relationship with the infiltration of macrophages. In addition, this study shows that the infiltration of mast cells, B-cells and macrophages varies depending on the degree of malignancy of neoplasia.
炎症细胞在肿瘤进展中发挥作用，具有预后和治疗潜力。分析了43例犬乳腺病变中肥大细胞类胰蛋白酶、巨噬细胞标志物、CD79a、IgA、IgM和IgG的免疫组化表达。在增生病变中，观察到少量B细胞（BCs）和肿瘤相关巨噬细胞（TAMs），而肿瘤相关肥大细胞（TAMCs）数量最多。在恶性病变的肿瘤周围基质中，TAMCs数量少，TAMAs和BCs数量多。每种类型的免疫细胞在肿瘤内总是低于肿瘤周围基质。在单纯癌和微乳头状癌的上皮细胞中也检测到CD79a阳性。免疫球蛋白反应主要位于上皮细胞，其中可检测到强烈的IgA和IgG阳性以及微弱的IgM阳性。根据我们的初步结果和文献数据，我们认为这些细胞和分子可能直接参与犬乳腺肿瘤的生物学过程。在乳腺癌中，基质炎症细胞和癌源性免疫球蛋白与肿瘤的进展、恶性程度和不良预后相关。本文报道的结果表明，犬乳腺上皮也表达免疫球蛋白，且它们大多与巨噬细胞浸润呈直接关系。此外，本研究表明肥大细胞、B细胞和巨噬细胞的浸润因肿瘤的恶性程度而异。
