Hughes D D
Food and Drug Administration, Kansas City, MO 64106.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):474-7.
A rapid method for the determination of dimetridazole and ipronidazole in feeds is described. The compounds are extracted from a borate buffer (pH 8.65) with benzene, partitioned into 1N HCl, and then partitioned back into benzene from a basic aqueous phase. The benzene extract is concentrated and injected onto a nonpolar (Apiezon L) gas chromatographic column for determination by 63Ni electron-capture detection. Recoveries from feeds of various composition, spiked at 0.2 ppm with both dimetridazole and ipronidazole, ranged from 70 to 115%; for the same feeds spiked at 1 ppm or more, the recoveries were greater than 80%. Carbadox, furazolidone, levamisole, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, arsanilic acid, piperazine, penicillin, and commonly added vitamins and minerals do not interfere. A 2-dimensional thin layer chromatographic system is presented as a means of additional identification.
本文描述了一种快速测定饲料中二甲硝唑和异丙硝唑的方法。这些化合物先用苯从硼酸盐缓冲液（pH 8.65）中萃取出来，再分配到1N盐酸中，然后又从碱性水相中重新分配回苯中。苯萃取液经浓缩后注入非极性（阿皮松L）气相色谱柱，用63Ni电子捕获检测器进行测定。在各种组成的饲料中添加0.2 ppm的二甲硝唑和异丙硝唑，回收率在70%至115%之间；对于添加量为1 ppm或更高的相同饲料，回收率大于80%。卡巴多司、呋喃唑酮、左旋咪唑、土霉素、金霉素、磺胺二甲嘧啶、磺胺喹恶啉、对氨基苯胂酸、哌嗪、青霉素以及常用的维生素和矿物质均不产生干扰。还介绍了一种二维薄层色谱系统作为辅助鉴定手段。
