Chalam R V, Stahr H M
J Assoc Off Anal Chem. 1979 May;62(3):570-2.
A thin layer chromatographic (TLC) method is described for the determination of citrinin in feeds. Citrinin is extracted from feed with methanol and water, the mixture is made alkaline with 10% sodium carbonate, and the aqueous solution is filtered and extracted with chloroform to remove most of the interfering materials. The aqueous layer is acidified with 2N HCl and extracted with chloroform. The chloroform extract is concentrated and spotted on a thin layer chromatographic (TLC) plate which is developed in chloroform-acetone-ethanol-water (60 + 40 + 10 + 1). The citrinin is viewed under ultraviolet light after TLC. Either visual or fluorodensitometric quantitation is used. Recoveries of citrinin from various feed samples spiked at levels of 2.0--5 micrograms/g were 75--92%. The proposed method can detect 0.5 micrograms/g feed, including corn, silage, ready mixed feeds, and feed pellets.
描述了一种用于测定饲料中桔青霉素的薄层色谱（TLC）方法。用甲醇和水从饲料中提取桔青霉素，用10%碳酸钠将混合物调至碱性，过滤水溶液并用氯仿萃取以去除大部分干扰物质。水层用2N盐酸酸化并用氯仿萃取。氯仿萃取液浓缩后点样于在氯仿 - 丙酮 - 乙醇 - 水（60 + 40 + 10 + 1）中展开的薄层色谱（TLC）板上。TLC后在紫外光下观察桔青霉素。采用目视或荧光光密度法定量。在2.0 - 5微克/克水平加标的各种饲料样品中桔青霉素的回收率为75 - 92%。所提出的方法可检测0.5微克/克的饲料，包括玉米、青贮饲料、预混饲料和饲料颗粒。
