Maeda Y, Ochi S, Masui T, Matubara S
Shizuoka Prefectural Institute of Public Health and Environmental Science, Japan.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):502-4.
The L-ascorbic acid (AsA) contents of candies and soft drinks available in the market were determined by liquid chromatography (LC). Samples are cleaned up on a disposable Sep-Pak C18 cartridge followed by reverse phase separation on an ODS column using a mobile phase of 0.1% phosphoric acid (pH 2.2). The AsA peak is detected on the basis of the UV absorption at 254 nm. The detection limit was 1 microgram/mL final concentration. Recoveries of AsA added at levels of 1-10 mg/g candy and 1-10 mg/10 mL soft drink were 99.2-101.7% with a coefficient of variation of 0.52-1.20% (n = 5). The present method allows rapid and accurate assays because it is a simple procedure compared with the official dye-titration method, and it is suitable for the routine analysis of AsA in selected candies and soft drinks.
采用液相色谱法（LC）测定了市售糖果和软饮料中L-抗坏血酸（AsA）的含量。样品先用一次性Sep-Pak C18柱净化，然后在ODS柱上进行反相分离，流动相为0.1%磷酸（pH 2.2）。根据254nm处的紫外吸收检测AsA峰。检测限为最终浓度1微克/毫升。在糖果中添加1-10毫克/克以及在软饮料中添加1-10毫克/10毫升AsA时，回收率为99.2-101.7%，变异系数为0.52-1.20%（n = 5）。与官方的染料滴定法相比，本方法操作简单，可实现快速准确的分析，适用于特定糖果和软饮料中AsA的常规分析。
