来自雪白根霉的一种碱基非特异性核糖核酸酶的一级结构。

Primary structure of a base non-specific ribonuclease from Rhizopus niveus.

作者信息

Horiuchi H, Yanai K, Takagi M, Yano K, Wakabayashi E, Sanda A, Mine S, Ohgi K, Irie M

机构信息

Department of Agricultural Chemistry, University of Tokyo.

出版信息

J Biochem. 1988 Mar;103(3):408-18. doi: 10.1093/oxfordjournals.jbchem.a122284.

DOI:10.1093/oxfordjournals.jbchem.a122284

PMID:3391995

Abstract

The primary structure of a base non-specific ribonuclease from Rhizopus niveus (RNase Rh) was determined by nucleotide sequence analysis of the DNA fragment encoding RNase Rh gene including signal peptide sequence, and amino acid sequence analysis of the peptide obtained from RNase Rh and RNase Rh' (a protease-modified RNase Rh created during the course of purification). The sequence determined was: MKAVLALATLIGSTLASSCSSTA LSCSNSANSDTCCSPEYGLVVLNMQWAPGYGPANAFTLHGLWPDKCSGAYAPSGGCDSN RASSSIASVIKSKDSSLYNSMLTYWPSNQGNNNVFWSHEWSKHGTCVSTYDPDCYDNYE EGEDIVDYFQKAMDLRSQYNVYKAFSSNGITPGGTYTATEMQSAIESYFGAKAKIDCSSG TLSDVALYFYVRGRDTYVITDALSTGSCSGDVEYPTK (the sequence of signal peptide is underlined). The sequence indicates that the homology with the sequence of RNase T2 from A. oryzae with the same base specificity is about 42% and that the sequences around the two histidine residues which are supposed to be involved in the active site are fairly conserved.

摘要

通过对编码包括信号肽序列的雪白根霉核糖核酸酶（RNase Rh）基因的DNA片段进行核苷酸序列分析，以及对从RNase Rh和RNase Rh'（纯化过程中产生的一种蛋白酶修饰的RNase Rh）获得的肽段进行氨基酸序列分析，确定了一种碱基非特异性核糖核酸酶的一级结构。所确定的序列为：MKAVLALATLIGSTLASSCSSTA LSCSNSANSDTCCSPEYGLVVLNMQWAPGYGPANAFTLHGLWPDKCSGAYAPSGGCDSN RASSSIASVIKSKDSSLYNSMLTYWPSNQGNNNVFWSHEWSKHGTCVSTYDPDCYDNYE EGEDIVDYFQKAMDLRSQYNVYKAFSSNGITPGGTYTATEMQSAIESYFGAKAKIDCSSG TLSDVALYFYVRGRDTYVITDALSTGSCSGDVEYPTK（信号肽序列加下划线）。该序列表明，与具有相同碱基特异性的米曲霉RNase T2序列的同源性约为42%，并且两个假定参与活性位点的组氨酸残基周围的序列相当保守。