Department of Nutrition, Texas A&M University, College Station, TX 77843, USA.
Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA.
Nutrients. 2021 Apr 10;13(4):1251. doi: 10.3390/nu13041251.
Various proteins or protein fractions reportedly positively affect gastrointestinal integrity and inflammation in diets providing >45% energy as fat. This study tested whether benefits were seen in diets providing 30% of energy as fat. Purified diets (PD) with isolated soy protein (ISP), dried whole milk powder (DWMP), milk fat globule membrane (MFGM), or milk protein concentrate (MPC) as protein sources were fed to C57BL/6J mice ( 15/diet group) for 13 weeks. MFGM-fed mice were heaviest ( < 0.005) but remained within breeder norms. Growth rates and gut motility were similar for all PD-fed mice. FITC-dextran assessed gut permeability was lowest in DWMP and MFGM ( = 0.054); overall, plasma endotoxin and unprovoked circulating cytokines indicated a non-inflammatory state for all PD-fed mice. Despite differences in cecal butyrate and intestinal gene expression, all PDs supported gastrointestinal health. Whole milk provided more positive effects compared to its fractions. However, ISP-fed mice showed a >370%, ( < 0.006) increase in colonic myeloperoxidase activity indicative of tissue neutrophil infiltration. Surprisingly, FITC-dextran and endotoxin outcomes were many folds better in PD-fed mice than mice (strain, vendor, age and sex matched) fed a "chow-type" nutritionally adequate non-PD. Additional variables within a diet's matrix appear to affect routine indicators or gastrointestinal health.
据报道，各种蛋白质或蛋白质片段在提供超过 45%能量作为脂肪的饮食中对胃肠道完整性和炎症有积极影响。本研究测试了在提供 30%能量作为脂肪的饮食中是否能看到益处。用分离的大豆蛋白（ISP）、全脂乳粉（DWMP）、乳脂肪球膜（MFGM）或乳蛋白浓缩物（MPC）作为蛋白质来源的纯化饮食（PD）喂给 C57BL/6J 小鼠（每组 15 只）13 周。MFGM 喂养的小鼠最重（<0.005），但仍在繁殖者的正常范围内。所有 PD 喂养的小鼠的生长速度和肠道蠕动都相似。FITC-右旋糖酐评估的肠道通透性在 DWMP 和 MFGM 中最低（=0.054）；总的来说，所有 PD 喂养的小鼠的血浆内毒素和无刺激循环细胞因子表明它们处于非炎症状态。尽管盲肠丁酸和肠道基因表达存在差异，但所有 PD 都支持胃肠道健康。全脂牛奶比其成分提供了更多的积极效果。然而，与 ISP 喂养的小鼠相比，喂食 PD 的小鼠的结肠髓过氧化物酶活性增加了>370%（<0.006），这表明组织中性粒细胞浸润。令人惊讶的是，与喂食"chow-type"（营养充足的非 PD）的匹配的小鼠（品系、供应商、年龄和性别）相比，喂食 PD 的小鼠的 FITC-右旋糖酐和内毒素结果要好得多。饮食基质中的其他变量似乎会影响常规指标或胃肠道健康。
