Ou Kuang-Ling, Wen Chia-Cheng, Lan Ching-Ya, Chen Yu-An, Wang Chih-Hsin, Wang Yi-Wen
Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan.
Division of Colon and Rectal Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan.
Life (Basel). 2021 Apr 17;11(4):350. doi: 10.3390/life11040350.
During ablative fractional resurfacing (AFR) laser therapy, thermal damage to the skin is inevitable, resulting in inflammatory responses and small wounds. Corticosteroids are known for their anti-inflammatory effect. However, inappropriate application of corticosteroids carries the risk of delayed wound healing. Therefore, we aimed to find the optimal administration route, timing, and duration of medium potency corticosteroid treatment to prevent AFR laser-induced inflammatory responses and to minimize the risk of delayed wound healing.
We determined the anti-inflammatory efficacy of corticosteroids by skin erythema and tissue biopsies on C57BL/6 mice. Wound healing was evaluated by crust area and epithelial gap. Finally, Masson's trichrome stain and α-SMA immunohistochemistry stain were used to analyze scar contracture.
Our results demonstrated that one dose of medium-potency topical corticosteroid applied immediately after AFR laser treatment could prevent erythema effectively with minimal disruption to wound healing. Notably, when more than one dose was administered, wound healing was delayed and scar contracture was aggravated by the application of medium-potency topical corticosteroids in a dosage-dependent manner.
Our findings suggested that single-dose medium-potency topical corticosteroids could potentially improve AFR laser-induced acute inflammatory responses in clinical applications.
在剥脱性分数激光皮肤重建（AFR）激光治疗期间，皮肤的热损伤不可避免，会引发炎症反应和小伤口。皮质类固醇以其抗炎作用而闻名。然而，皮质类固醇的不当使用存在伤口愈合延迟的风险。因此，我们旨在找到中效皮质类固醇治疗的最佳给药途径、时机和持续时间，以预防AFR激光引起的炎症反应，并将伤口愈合延迟的风险降至最低。
我们通过对C57BL/6小鼠的皮肤红斑和组织活检来确定皮质类固醇的抗炎效果。通过结痂面积和上皮间隙评估伤口愈合情况。最后，使用Masson三色染色和α-SMA免疫组织化学染色分析瘢痕挛缩。
我们的结果表明，AFR激光治疗后立即应用一剂中效外用皮质类固醇可有效预防红斑，对伤口愈合的干扰最小。值得注意的是，当给药超过一剂时，中效外用皮质类固醇的应用会以剂量依赖的方式延迟伤口愈合并加重瘢痕挛缩。
我们的研究结果表明，单剂量中效外用皮质类固醇在临床应用中可能改善AFR激光引起的急性炎症反应。
