Wojnicz Dorota, Tichaczek-Goska Dorota, Gleńsk Michał, Hendrich Andrzej B
Department of Biology and Medical Parasitology, Wrocław Medical University, 50-367 Wrocław, Poland.
Department of Pharmacognosy and Herbal Medicines, Wrocław Medical University, 50-367 Wrocław, Poland.
Pharmaceutics. 2021 Apr 17;13(4):573. doi: 10.3390/pharmaceutics13040573.
European goldenrod ( L.) has long been applied in traditional medicine and recommended in the prophylaxis of urinary tract infections (UTIs). However, research describing the antibacterial properties of goldenrod is very limited. Therefore, the aim of the study was to determine the effect of extract on the survival and biofilm formation of uropathogenic . The interactions between the goldenrod extract and antibiotics used in UTIs were established. The influence of the extract on the duration of the post-antibiotic effects (PAE) and post-antibiotic sub-MIC effects (PASME) of amikacin and ciprofloxacin were determined. Extract composition was analyzed using coupled UHPLC/MS and the spectrophotometric method. The survival of bacteria was established using the serial dilution assay. The crystal violet assay for biofilm quantification was also used. PAE and PASME were investigated using the viable count method. The obtained results indicate that extract limits the survival of planktonic forms of bacteria and reduces 24-h biofilm. However, the combination of extract with antibiotics weakens their antibacterial activity and shortens the duration of PAE and PASME. Therefore, when deciding to use a combination of extract and amikacin/ciprofloxacin, it is necessary to take into account their antagonistic activity.
欧洲一枝黄花长期以来一直应用于传统医学，并被推荐用于预防尿路感染（UTIs）。然而，描述一枝黄花抗菌特性的研究非常有限。因此，本研究的目的是确定[提取物名称]提取物对尿路致病性[细菌名称]的存活和生物膜形成的影响。建立了一枝黄花提取物与UTIs中使用的抗生素之间的相互作用。测定了提取物对阿米卡星和环丙沙星的抗生素后效应（PAE）和抗生素后亚抑菌浓度效应（PASME）持续时间的影响。使用超高效液相色谱/质谱联用和分光光度法分析提取物成分。使用系列稀释法确定细菌的存活率。还使用结晶紫测定法对生物膜进行定量。使用活菌计数法研究PAE和PASME。所得结果表明，[提取物名称]提取物限制了浮游形式细菌的存活并减少了24小时生物膜。然而，[提取物名称]提取物与抗生素的组合会削弱它们的抗菌活性并缩短PAE和PASME的持续时间。因此，在决定使用[提取物名称]提取物与阿米卡星/环丙沙星的组合时，有必要考虑它们的拮抗活性。
