Rył Bartosz, Jasicka-Misiak Izabela
Institute of Chemistry, University of Opole, 48 Oleska Str., 45-052 Opole, Poland.
Molecules. 2025 Jun 26;30(13):2746. doi: 10.3390/molecules30132746.
Chymotrypsin inhibitors were initially considered mainly as anti-nutritional factors. However, the potential for their use as therapeutics has been recognized, particularly in the control of cancer, neurodegenerative diseases, and inflammatory processes. The search for new, effective, and safe chymotrypsin inhibitors has become important not only for food and feed safety reasons, but also in the search for new compounds with potential for use in the pharmaceutical industry. Oxidative stress is also an integral etiological factor in the development of the aforementioned pathological conditions. Antioxidants supplied with food can have an impact on reducing the probability of developing these diseases. Herbaceous plants are a valuable reservoir of biologically active chemical compounds, which can show both inhibitory effects against a number of enzymatic reactions and have antioxidant activity. The compounds found within them are also often characterized by higher bioavailability and safety than their synthetic analogs. In the present study, phytochemical characterization of plant materials L., L., L. and L. was performed, in order to search for new, potential substances with chymotrypsin inhibitor and antioxidant properties. Antioxidant and inhibitory activities against chymotrypsin were determined using effect-directed HPTLC. The total content of phenolic compounds and flavonoids and antioxidant activity were also determined in UV-Vis spectrophotometric tests. Both plant species showed antioxidant and chymotrypsin inhibitory activity. Among the methanol and methanol:water extracts, the extracts from sp. showed stronger inhibitory and antioxidant activity. However, in the case of dichloromethane extracts, inhibited chymotrypsin activity in a stronger manner than sp. The results indicate the application potential of compounds obtained from these plants as chymotrypsin inhibitors and antioxidant agents.
胰凝乳蛋白酶抑制剂最初主要被视为抗营养因子。然而，人们已经认识到它们作为治疗剂的潜力，特别是在控制癌症、神经退行性疾病和炎症过程方面。寻找新的、有效且安全的胰凝乳蛋白酶抑制剂不仅出于食品和饲料安全的原因变得很重要，而且在寻找有潜力用于制药行业的新化合物方面也很重要。氧化应激也是上述病理状况发展过程中不可或缺的病因学因素。食物中提供的抗氧化剂可以对降低患这些疾病的概率产生影响。草本植物是生物活性化合物的宝贵来源，这些化合物既可以对许多酶促反应表现出抑制作用，又具有抗氧化活性。它们所含的化合物通常还具有比其合成类似物更高的生物利用度和安全性。在本研究中，对植物材料L.、L.、L.和L.进行了植物化学表征，以寻找具有胰凝乳蛋白酶抑制和抗氧化特性的新的潜在物质。使用效应导向的高效薄层色谱法测定了对胰凝乳蛋白酶的抗氧化和抑制活性。还通过紫外可见分光光度法测定了酚类化合物和黄酮类化合物的总含量以及抗氧化活性。两种植物都表现出抗氧化和胰凝乳蛋白酶抑制活性。在甲醇提取物和甲醇:水提取物中，sp.的提取物表现出更强的抑制和抗氧化活性。然而，在二氯甲烷提取物的情况下，对胰凝乳蛋白酶活性的抑制作用比sp.更强。结果表明从这些植物中获得的化合物作为胰凝乳蛋白酶抑制剂和抗氧化剂的应用潜力。
