The Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2021 Apr 15;22(8):4069. doi: 10.3390/ijms22084069.
Rice false smut is a fungal disease distributed worldwide and caused by . In this study, we identified a putative ester cyclase (named as UvEC1) as being significantly upregulated during infection. UvEC1 contained a SnoaL-like polyketide cyclase domain, but the functions of ketone cyclases such as SnoaL in plant fungal pathogens remain unclear. Deletion of caused defects in vegetative growth and conidiation. was also required for response to hyperosmotic and oxidative stresses and for maintenance of cell wall integrity. Importantly, Δ mutants exhibited reduced virulence. We performed a tandem mass tag (TMT)-based quantitative proteomic analysis to identify differentially accumulating proteins (DAPs) between the Δ mutant and the wild-type isolate HWD-2. Proteomics data revealed that UvEC1 has a variety of effects on metabolism, protein localization, catalytic activity, binding, toxin biosynthesis and the spliceosome. Taken together, our findings suggest that is critical for the development and virulence of .
水稻纹枯病是一种世界性分布的真菌病害，由 引起。本研究中，我们鉴定出一个假定的酯环化酶（命名为 UvEC1）在 感染过程中显著上调。UvEC1 含有一个 SnoaL 样聚酮合酶结构域，但植物真菌病原体中 SnoaL 等酮合酶的功能仍不清楚。缺失 导致营养生长和产孢缺陷。还需要对高渗和氧化应激作出反应，并维持细胞壁的完整性。重要的是，Δ 突变体表现出降低的毒力。我们进行了串联质量标签（TMT）定量蛋白质组学分析，以鉴定 Δ 突变体与野生型分离株 HWD-2 之间差异积累的蛋白质（DAPs）。蛋白质组学数据显示，UvEC1 对代谢、蛋白质定位、催化活性、结合、毒素生物合成和剪接体有多种影响。总之，我们的研究结果表明， 在 的发育和毒力中是至关重要的。
