Tytgat Institute for Liver and Intestinal Research, Amsterdam Gastroenterology Endocrinology and Metabolism, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, 1105 BK Amsterdam, The Netherlands.
Department of Vascular Medicine, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Nutrients. 2021 Apr 15;13(4):1305. doi: 10.3390/nu13041305.
β-glucan consumption is known for its beneficial health effects, but the mode of action is unclear. While humans and mice lack the required enzymes to digest β-glucans, certain intestinal microbes can digest β-glucans, triggering gut microbial changes. Curdlan, a particulate β-glucan isolated from , is used as a food additive. In this study we determined the effect of curdlan intake in mice on the intestinal microbiota and dextran sodium sulfate (DSS)-induced intestinal inflammation. The effect of curdlan on the human intestinal microbiota was assessed using i-screen, an assay for studying anaerobic microbial interactions. Mice received oral gavage with vehicle or curdlan for 14 days followed by DSS for 7 days. The curdlan-fed group showed reduced weight loss and colonic inflammation compared to the vehicle-fed group. Curdlan intake did not induce general microbiota community changes, although a specific , closely related to , was observed to be 10- to 100-fold more prevalent in the curdlan-fed group under control and colitis conditions, respectively. When tested in i-screen, curdlan induced a global change in the microbial composition of the healthy intestinal microbiota from a human. Overall, these results suggest that dietary curdlan induces microbiota changes that could reduce intestinal inflammation.
β-葡聚糖的摄入对健康有益，但其作用机制尚不清楚。虽然人类和小鼠缺乏消化β-葡聚糖所需的酶，但某些肠道微生物可以消化β-葡聚糖，从而引发肠道微生物的变化。从 中分离出的颗粒状β-葡聚糖——结冷胶，被用作食品添加剂。在这项研究中，我们确定了小鼠摄入结冷胶对肠道微生物群和葡聚糖硫酸钠（DSS）诱导的肠道炎症的影响。使用 i-screen 评估了结冷胶对人类肠道微生物群的影响，i-screen 是一种用于研究厌氧微生物相互作用的检测方法。小鼠接受口服灌胃 14 天，分别用载体或结冷胶喂养，然后用 DSS 喂养 7 天。与载体喂养组相比，结冷胶喂养组的体重减轻和结肠炎症减少。结冷胶摄入并没有引起一般微生物群落的变化，尽管在对照和结肠炎条件下，一种与 密切相关的 ，分别在结冷胶喂养组中观察到 10-100 倍更为普遍。在 i-screen 中进行测试时，结冷胶诱导了来自人类的健康肠道微生物群落的微生物组成的全局变化。总的来说，这些结果表明，饮食中的结冷胶会引起微生物群的变化，从而减少肠道炎症。
