血浆和唾液中咖啡因浓度的高效液相色谱分析。

High performance liquid chromatographic analysis of caffeine concentrations in plasma and saliva.

作者信息

Alkaysi H N, Salem M S, el-Sayed Y M

机构信息

Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.

出版信息

J Clin Pharm Ther. 1988 Apr;13(2):109-15. doi: 10.1111/j.1365-2710.1988.tb00165.x.

DOI:10.1111/j.1365-2710.1988.tb00165.x

PMID:3392124

Abstract

A rapid high performance liquid chromatographic (HPLC) method for the analysis of caffeine in plasma and saliva is described. Samples of saliva and plasma were purified using zinc sulphate solution as protein precipitant. The supernatant was injected directly onto the column. The mobile phase consisted of ammonium acetate buffer:acetonitrile:methanol (82:15:3, v/v). Measurements were carried out at 254 nm. Acetanilide was used as the internal standard and analysis was completed in 10 min. No interference from endogenous components or other methylxanthines was observed. The coefficients of variation for within day and between day analysis for both saliva and plasma were less than 7.66%. Samples were collected from 20 volunteers. The correlation coefficient between plasma and saliva caffeine concentrations was found to be 0.98.

摘要

描述了一种用于分析血浆和唾液中咖啡因的快速高效液相色谱（HPLC）方法。使用硫酸锌溶液作为蛋白质沉淀剂对唾液和血浆样品进行纯化。将上清液直接注入色谱柱。流动相由醋酸铵缓冲液：乙腈：甲醇（82:15:3，v/v）组成。在254nm处进行测定。使用乙酰苯胺作为内标，10分钟内完成分析。未观察到内源性成分或其他甲基黄嘌呤的干扰。唾液和血浆日内和日间分析的变异系数均小于7.66%。从20名志愿者中采集样品。发现血浆和唾液咖啡因浓度之间的相关系数为0.98。