Pichini S, Altieri I, Pacifici R, Rosa M, Zuccaro P
Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
J Chromatogr. 1991 Jul 17;568(1):267-9. doi: 10.1016/0378-4347(91)80363-h.
A high-performance liquid chromatographic method with ultraviolet photometric detection for the determination of cotinine in human plasma was described. The use of a 30-cm reversed-phase column and of a mobile phase consisting of water-methanol-0.1 M sodium acetate-acetonitrile (72:21:5.6:1.4, v/v), pH 4.1, eliminated caffeine interference. A simplified solid-phase extraction procedure was also performed for plasma samples.
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