高效液相色谱法测定人血浆中可替宁时咖啡因干扰的消除
Elimination of caffeine interference in high-performance liquid chromatographic determination of cotinine in human plasma.
作者信息
Pichini S, Altieri I, Pacifici R, Rosa M, Zuccaro P
机构信息
Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
出版信息
J Chromatogr. 1991 Jul 17;568(1):267-9. doi: 10.1016/0378-4347(91)80363-h.
A high-performance liquid chromatographic method with ultraviolet photometric detection for the determination of cotinine in human plasma was described. The use of a 30-cm reversed-phase column and of a mobile phase consisting of water-methanol-0.1 M sodium acetate-acetonitrile (72:21:5.6:1.4, v/v), pH 4.1, eliminated caffeine interference. A simplified solid-phase extraction procedure was also performed for plasma samples.
描述了一种采用紫外光度检测的高效液相色谱法测定人血浆中可替宁的方法。使用一根30厘米的反相柱以及由水 - 甲醇 - 0.1M醋酸钠 - 乙腈（72:21:5.6:1.4，v/v）组成、pH值为4.1的流动相，消除了咖啡因的干扰。还对血浆样品进行了简化的固相萃取程序。
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