Masaka Edmore, Reed Sue, Davidson Maggie, Oosthuizen Jacques
Public Health and Occupational Health and Safety, School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA 6027, Australia.
Pathogens. 2021 Apr 12;10(4):462. doi: 10.3390/pathogens10040462.
Water mist systems (WMS) are used for evaporative cooling in public areas. The health risks associated with their colonization by opportunistic premise plumbing pathogens (OPPPs) is not well understood. To advance the understanding of the potential health risk of OPPPs in WMS, biofilm, water and bioaerosol samples (n = 90) from ten (10) WMS in Australia were collected and analyzed by culture and polymerase chain reaction () methods to detect the occurrence of five representative OPPPs: , , , and . (44%, n = 90) occurred more frequently in samples, followed by serogroup (Sg) 2-14 (18%, n = 90) and Sg 1 (6%, n = 90). A negative correlation between OPPP occurrence and residual free chlorine was observed except with , (30) = 0.067, > 0.05. All detected OPPPs were positively correlated with total dissolved solids (TDS) except with . Biofilms contained higher concentrations of Sg 2-14 (1000-3000 CFU/mL) than water samples (0-100 CFU/mL). This study suggests that WMS can be colonized by OPPPs and are a potential health risk if OPPP contaminated aerosols get released into ambient atmospheres.
水雾系统(WMS)用于公共场所的蒸发冷却。人们对机会性建筑物管道病原体(OPPPs)在该系统中定殖所带来的健康风险了解不足。为了加深对OPPPs在WMS中潜在健康风险的认识,采集了澳大利亚10个WMS的生物膜、水和生物气溶胶样本(n = 90),并通过培养和聚合酶链反应()方法进行分析,以检测5种代表性OPPPs的存在情况:、、、和。(44%,n = 90)在样本中出现的频率更高,其次是血清群(Sg)2 - 14(18%,n = 90)和Sg 1(6%,n = 90)。除了与,(30)= 0.067,> 0.05外,观察到OPPPs的出现与残余游离氯之间呈负相关。除了与外,所有检测到的OPPPs与总溶解固体(TDS)呈正相关。生物膜中血清群2 - 14(1000 - 3000 CFU/mL)的浓度高于水样(0 - 100 CFU/mL)。这项研究表明,WMS可能会被OPPPs定殖,如果被OPPPs污染的气溶胶释放到周围大气中,会存在潜在的健康风险。