Sundermann Julius, Sydow Steffen, Burmeister Laura, Hoffmann Andrea, Menzel Henning, Bunjes Heike
Technische Universität Braunschweig, Institut für Pharmazeutische Technologie und Biopharmazie, 38106 Braunschweig, Germany.
Technische Universität Braunschweig, Institut für Technische Chemie, 38106 Braunschweig, Germany.
Pharmaceutics. 2021 Apr 19;13(4):582. doi: 10.3390/pharmaceutics13040582.
Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable release results, the robustness of a commercially available ELISA for -derived BMP-2 and the parallel determination of BMP-2 recovery using a quantitative biological activity assay were investigated within a common release setup, with special reference to solubility and matrix effects. Without bovine serum albumin and Tween 20 as solubilizing additives to release media buffed at physiological pH, BMP-2 recoveries after release were notably reduced. In contrast, the addition of chitosan to release samples caused an excessive recovery. A possible explanation for these effects is the reversible aggregation tendency of BMP-2, which might be influenced by an interaction with chitosan. The interfering effects highlighted in this study are of great importance for bio-assay-based BMP-2 quantification, especially in the context of pharmaceutical release experiments.
具有骨形态发生蛋白2（BMP-2）定制释放功能的壳聚糖纳米凝胶涂层聚己内酯（PCL）纤维垫基植入物原型，是实现植入物介导骨再生的一种有前景的方法。为确保可靠的释放结果，在常见的释放设置中研究了一种市售ELISA检测释放的BMP-2的稳健性，并使用定量生物活性测定法平行测定BMP-2的回收率，特别参考了溶解度和基质效应。在生理pH值缓冲的释放介质中，若不添加牛血清白蛋白和吐温20作为增溶添加剂，释放后BMP-2的回收率显著降低。相反，向释放样品中添加壳聚糖会导致回收率过高。这些效应的一个可能解释是BMP-2的可逆聚集倾向，这可能受其与壳聚糖相互作用的影响。本研究中突出的干扰效应对于基于生物测定的BMP-2定量非常重要，尤其是在药物释放实验的背景下。
