Institute for Technical Chemistry, Braunschweig University of Technology, Hagenring 30, 38106, Braunschweig, Germany.
Institute for Inorganic Chemisty, Leibniz University of Hannover, Callinstr. 9, 30167, Hannover, Germany; NIFE - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Stadtfelddamm 34, 30625, Hannover, Germany.
Colloids Surf B Biointerfaces. 2018 Mar 1;163:309-320. doi: 10.1016/j.colsurfb.2017.12.050. Epub 2017 Dec 29.
Electrospun poly(ε-caprolactone) (PCL) fiber mats are modified using a chitosan grafted with PCL (CS-g-PCL), to improve the biological performance and to enable further modifications. The graft copolymer is immobilized by the crystallization of the PCL grafts on the PCL fiber surface as binding mechanism. In this way, the surface of the fibers is covered with chitosan bearing cationic amino groups, which allow adsorption of oppositely charged nanoparticulate drug-delivery systems. The modification of the fiber mats and the attachment of the drug delivery systems are easy and scalable dip processes. The process is also versatile; it is possible to attach different polymeric and inorganic nanoparticulate drug-release systems of cationic or anionic nature. The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). As proof of principle, the release of ciprofloxacin from silica nanoparticles attached to the modified fiber mats is shown; however, the method is also suited for other biologically active substances including growth factors. The initial cellular attachment and proliferation as well as vitality of the cells is improved by the modification with CS-g-PCL and is further influenced by the type of the drug delivery system attached. Hence, this method can be used to transfer PCL fiber mats into bioactive implants for in-situ tissue engineering applications.
静电纺丝聚（ε-己内酯）（PCL）纤维垫用接枝了 PCL 的壳聚糖（CS-g-PCL）进行改性，以改善生物性能并实现进一步的改性。接枝共聚物通过 PCL 接枝在 PCL 纤维表面上的结晶作为结合机制而固定。这样，纤维表面就被带有正电荷氨基的壳聚糖覆盖，这允许吸附带相反电荷的纳米颗粒药物输送系统。纤维垫的改性和药物输送系统的附着是容易和可扩展的浸涂工艺。该工艺也具有多功能性；可以附着不同的聚合物和无机纳米颗粒阳离子或阴离子性质的药物释放系统。使用扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）验证了修饰。作为原理的证明，展示了附着在改性纤维垫上的硅纳米颗粒中环丙沙星的释放；然而，该方法也适用于包括生长因子在内的其他生物活性物质。用 CS-g-PCL 进行改性可以提高细胞的初始附着、增殖和活力，并且附着的药物输送系统的类型也会进一步影响细胞的活力。因此，该方法可用于将 PCL 纤维垫转化为用于原位组织工程应用的生物活性植入物。
