School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Guangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, China.
Molecules. 2021 Apr 23;26(9):2464. doi: 10.3390/molecules26092464.
In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs). However, the absence of in vivo trials undermines these results. This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats. Twelve Sprague-Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (), and the control group was treated with normal water for five weeks. Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as and , and decreased levels of pathogens, including . Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis. Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid. Moreover, the concentrations of propionate and butyrate were elevated in the rats administered with isomaltulose. This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.
体外实验表明异麦芽酮糖具有益生元活性，能刺激益生菌的生长和短链脂肪酸（SCFAs）的产生。然而，由于缺乏体内试验，这些结果仍有待证实。本研究旨在探讨异麦芽酮糖对大鼠肠道微生物群组成和功能的影响。将 12 只 Sprague-Dawley 大鼠分为两组：异麦芽酮糖组自由饮用含 10%异麦芽酮糖（）的水，对照组则用普通水进行为期五周的处理。此外，16S rRNA 测序显示，摄入异麦芽酮糖增加了有益菌的丰度，如和，同时降低了病原体的水平，包括。细菌功能预测表明，异麦芽酮糖影响肠道微生物功能，包括次级胆汁酸生物合成。靶向代谢组学表明，异麦芽酮糖补充剂增加了胆酸的浓度，同时降低了石胆酸、脱氧胆酸、去氧胆酸和羊去氧胆酸的水平。此外，给予异麦芽酮糖的大鼠体内丙酸和丁酸的浓度也升高了。本研究表明，异麦芽酮糖可调节大鼠肠道微生物群和 SCFAs 及次级胆汁酸的产生，为异麦芽酮糖作为益生元的应用提供了科学依据。
