Diagnostic Techniques Unit, Collegium Maximum, Medical University of Lublin, Staszica 4/6, 20-081 Lublin, Poland.
Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
Cells. 2021 Apr 25;10(5):1013. doi: 10.3390/cells10051013.
Immune modulatory factors like indoleamine 2,3-dioxygenase 1 (IDO1) generating kynurenine (Kyn) and receptor for advanced glycation end-products (RAGE) contribute to endometrial and cancer microenvironment. Using adequate experimental models is needed to learn about the significance of these molecular factors in endometrial biology. In this paper we study IDO1 activity and RAGE expression in the in vitro cultured primary human endometrial cells derived from cancerous and noncancerous tissue.
The generated primary cell cultures from cancer and noncancerous endometrial tissues were characterized using immunofluorescence and Western Blot for expression of endometrial and cancer markers. IDO1 activity was studied by Kyn quantification with High Performance Liquid Chromatography with Diode Array Detector.
The primary cultures of endometrial cells were obtained with 80% success rate and no major genetic aberrations. The cells retained in vitro expression of markers (mucin MUC1 and HER2) or immunomodulatory factors (RAGE and IDO1). Increased Kyn secretion was associated with cancer endometrial cell culture in contrast to the control one.
Primary endometrial cells express immune modulatory factors RAGE and IDO1 in vitro associated with cancer phenotype of endometrium.
吲哚胺 2,3-双加氧酶 1（IDO1）生成犬尿氨酸（Kyn）和晚期糖基化终产物受体（RAGE）等免疫调节因子有助于子宫内膜和肿瘤微环境。需要使用适当的实验模型来了解这些分子因素在子宫内膜生物学中的意义。在本文中，我们研究了体外培养的源自癌组织和非癌组织的原发性人子宫内膜细胞中 IDO1 活性和 RAGE 表达。
通过免疫荧光和 Western Blot 对子宫内膜和癌症标志物的表达对源自癌组织和非癌组织的生成的原代细胞培养物进行了表征。通过带有二极管阵列检测器的高效液相色谱法对 Kyn 进行定量来研究 IDO1 活性。
子宫内膜细胞的原代培养物的成功率为 80%，且没有主要的遗传异常。细胞在体外保留了标志物（黏蛋白 MUC1 和 HER2）或免疫调节因子（RAGE 和 IDO1）的表达。与对照相比，癌细胞子宫内膜细胞培养物与 Kyn 分泌增加相关。
体外培养的原发性子宫内膜细胞表达免疫调节因子 RAGE 和 IDO1，与子宫内膜的癌症表型相关。
